ZFIN ID: ZDB-FIG-190618-2
Young et al., 2019 - Compensatory growth renders Tcf7l1a dispensable for eye formation despite its requirement in eye field specification. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Observed In:
Stage: Bud

Fig. 2

The prospective forebrain and eye field domains of the neural plate are reduced in Ztcf7l1a-/- mutants.

(A) Graph showing RT-qPCR quantification of the mRNA levels of lef1tcf7tcf7l1a, tcf7l1btcf7l2otx1botx2six3b and rx3 in Ztcf7l1a-/- mutants relative to wildtype embryos at 10hpf. Biological and technical triplicates, two independent experiments. (B, C) Quantification of the forebrain domain of the anterior neural plate (B) enclosed by emx3 up to pax2a (D, E) expression by in situ hybridisation (reduction to an average of 76%, n = 11, one experiment, data in Supplementary file 1B), and eye field volume (C) by rx3 fluorescent in situhybridisation confocal volume reconstruction (J–M) (reduction to an average of 55%, n = 10, one experiment, data in Supplementary file 1C). (D–I) Expression of emx3 (arrowhead)/pax2a(D, E), six3b (arrowhead)/pax2a (F, G) and rx3 (arrowhead)/pax2a (H, I) in wildtype (D, F, H) and Ztcf7l1a-/- (E, G, I) embryos detected by in situ hybridisation at 10hpf. Reduction of six3band rx3 expression 100%, n > 40, three experiments. (J–M) Confocal volume reconstruction of rx3 fluorescent in situ hybridisation in wildtype (J, K) and Ztcf7l1a-/- (L, M) mutants at 10hpf. (J, L) Dorsal view, anterior to top, and (K, M) transverse view from posterior, dorsal up. Abbreviations: mb, midbrain; pcp, prechordal plate Scale Bars = 250 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
emx3 WT standard conditions Bud anterior neural keel ISH
tcf7l1am881/m881 standard conditions Bud anterior neural keel ISH
lef1 tcf7l1am881/m881 standard conditions Bud whole organism RTPCR
otx1 tcf7l1am881/m881 standard conditions Bud whole organism RTPCR
otx2b tcf7l1am881/m881 standard conditions Bud whole organism RTPCR
pax2a WT standard conditions Bud midbrain neural keel ISH
tcf7l1am881/m881 standard conditions Bud midbrain neural keel ISH
rx3 WT standard conditions Bud forebrain neural keel ISH
tcf7l1am881/m881 standard conditions Bud forebrain neural keel ISH
Bud whole organism RTPCR
six3b WT standard conditions Bud forebrain neural keel ISH
tcf7l1am881/m881 standard conditions Bud forebrain neural keel ISH
Bud whole organism RTPCR
tcf7 tcf7l1am881/m881 standard conditions Bud whole organism RTPCR
tcf7l1a tcf7l1am881/m881 standard conditions Bud whole organism RTPCR
tcf7l1b tcf7l1am881/m881 standard conditions Bud whole organism RTPCR
tcf7l2 tcf7l1am881/m881 standard conditions Bud whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tcf7l1am881/m881 standard conditions Bud forebrain decreased size, abnormal
Bud forebrain neural keel rx3 expression decreased amount, abnormal
Bud forebrain neural keel six3b expression decreased amount, abnormal
Bud optic primordium decreased size, abnormal
Bud whole organism rx3 expression decreased amount, abnormal
Bud whole organism six3b expression decreased amount, abnormal
Bud whole organism tcf7l1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife