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ZFIN ID: ZDB-FIG-181024-5
Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-25

Fig. 1

Combinatorial signaling establishes distinct Fox expression domains. (A) In situ hybridization at 36 hpf shows expression of Fox genes (green) relative to dlx2a+ CNCs (red) of the first two arches. (B) Expression of foxc1a, foxd2 and foxf1 (green) relative to dlx2a+ CNCs (red) upon misexpression of Shha, Bmp4 or Fgf3. Embryos doubly transgenic for hsp70l:Gal4 and UAS:shha, UAS:bmp4 or UAS:fgf3 were subjected to heat shock induction from 20 to 24 hpf and then fixed at 36 hpf for analysis. (C) Expression of foxc1a, foxd2 and foxf1 (green) relative to dlx2a+ CNCs (red) in edn1 or jag1b mutants versus sibling controls. Numbers denote proportions of embryos with displayed patterns. Scale bars: 25 μm. (D) Summary of positive and negative inputs on Fox expression in the first two arches.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
kca4Tg ; el688Tg heat shock Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
foxc1a WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-25 Not Detected pharyngeal arch 1 ISH
Prim-25 Not Detected pharyngeal arch 2 ISH
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el49Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch 1 ISH
Prim-25 Not Detected pharyngeal arch 2 ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
kca4Tg ; el688Tg heat shock Prim-25 Not Detected pharyngeal arch 1 ISH
Prim-25 Not Detected pharyngeal arch 2 ISH
foxc1b WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxd1 WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxd2 WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el49Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch 1 ISH
Prim-25 Not Detected pharyngeal arch 2 ISH
jag1bb1105/b1105 standard conditions Prim-25 Not Detected pharyngeal arch 1 ISH
Prim-25 Not Detected pharyngeal arch 2 ISH
kca4Tg ; el688Tg heat shock Prim-25 Not Detected pharyngeal arch 1 ISH
Prim-25 Not Detected pharyngeal arch 2 ISH
foxf1 WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
kca4Tg ; el688Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxf2a WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxf2b WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxl1 WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 foxc1a expression absent, abnormal
Prim-25 pharyngeal arch 1 foxf1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 foxd2 expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 foxc1a expression absent, abnormal
Prim-25 pharyngeal arch 2 foxd2 expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 foxf1 expression spatial pattern, abnormal
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 foxc1a expression increased amount, abnormal
Prim-25 pharyngeal arch 1 foxd2 expression increased amount, abnormal
Prim-25 pharyngeal arch 1 foxf1 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 foxd2 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 foxf1 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1a expression increased amount, abnormal
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 foxc1a expression absent, abnormal
Prim-25 pharyngeal arch 1 foxd2 expression absent, abnormal
Prim-25 pharyngeal arch 1 foxf1 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1a expression absent, abnormal
Prim-25 pharyngeal arch 2 foxd2 expression absent, abnormal
Prim-25 pharyngeal arch 2 foxf1 expression increased amount, abnormal
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 foxd2 expression absent, abnormal
Prim-25 pharyngeal arch 2 foxd2 expression absent, abnormal
kca4Tg ; el688Tg heat shock Prim-25 pharyngeal arch 1 foxd2 expression absent, abnormal
Prim-25 pharyngeal arch 1 foxc1a expression absent, abnormal
Prim-25 pharyngeal arch 1 foxf1 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1a expression absent, abnormal
Prim-25 pharyngeal arch 2 foxd2 expression absent, abnormal
Prim-25 pharyngeal arch 2 foxf1 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development