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ZFIN ID: ZDB-FIG-181024-12
Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-25

Fig. S2

Regulation of foxc1b and foxd1 arch expression

Expression of foxc1b (A) and foxd1 (B) in green, relative to dlx2a+ CNCs (red) of the first two arches. Similar to foxc1a in Figure 1, foxc1b and foxd1 expression is expanded by Shha misexpression, inhibited by Bmp4 and Fgf3 misexpression, and reduced in edn1 but not jag1b mutants. Embryos doubly transgenic for hsp70l:Gal4 and UAS:shha, UAS:bmp4, or UAS:fgf3 were subjected to a heatshock from 20-24 hpf to induce ligand expression throughout embryos. Numbers indicate proportion of animals showing the displayed patterns. Scale bars = 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
kca4Tg ; el688Tg heat shock Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
foxc1b edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
kca4Tg ; el688Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxd1 edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
kca4Tg ; el688Tg heat shock Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch 1 foxc1b expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 foxd1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 foxd1 expression decreased distribution, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1b expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression decreased distribution, abnormal
el137Tg ; kca4Tg heat shock Prim-25 pharyngeal arch 1 foxd1 expression increased amount, abnormal
Prim-25 pharyngeal arch 1 foxc1b expression increased amount, abnormal
Prim-25 pharyngeal arch 1 foxd1 expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 foxc1b expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1b expression increased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1b expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression increased distribution, abnormal
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 foxc1b expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 foxd1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 foxd1 expression decreased distribution, abnormal
Prim-25 pharyngeal arch 1 foxc1b expression decreased distribution, abnormal
Prim-25 pharyngeal arch 2 foxc1b expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1b expression decreased distribution, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression decreased distribution, abnormal
kca4Tg ; el688Tg heat shock Prim-25 pharyngeal arch 1 foxd1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 foxc1b expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 foxd1 expression decreased distribution, abnormal
Prim-25 pharyngeal arch 1 foxc1b expression decreased distribution, abnormal
Prim-25 pharyngeal arch 2 foxc1b expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 foxc1b expression decreased distribution, abnormal
Prim-25 pharyngeal arch 2 foxd1 expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development