ZFIN ID: ZDB-FIG-150916-37
Patil et al., 2015 - scaRNAs Regulate Splicing and Vertebrate Heart Development. Biochimica et biophysica acta. Molecular basis of disease   1852(8):1619-29 Full text @ BBA Molecular Basis of Disease
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 8

Splice isoform changes after targeting scarna1. Treating zebrafish embryos with anti-scarna1 morpholino causes changes in exon retention of cardiac regulatory genes. 13 of 39 members of the Wnt family have significant changes in exon retention after treatment with antisense morpholino (assessed by RNA-Seq and qRT-PCR, values shown are from qRT-PCR data quantifying the variable exon). The misMO oligo is a negative control that differs from the primary morpholino by 5 nucleotides in the critical binding site. The gene name is shown on the horizontal axis and the numbers represent the start position of the exon. *Significantly different from the WT, p < 0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dot1l AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
fzd3b AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
kctd15a AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
mllt10 AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
sfrp1a AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
vangl2 AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
wnt4 AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
wnt4b AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
wnt8a AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
wnt8b AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
wnt10a AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
wnt10b AB control Prim-5 whole organism RTPCR
AB + MO1-scarna1 standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-scarna1 standard conditions Prim-5 regulation of alternative mRNA splicing, via spliceosome disrupted, abnormal
AB + MO1-snord94 standard conditions Prim-5 regulation of alternative mRNA splicing, via spliceosome disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biochimica et biophysica acta. Molecular basis of disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Biochimica et biophysica acta. Molecular basis of disease, 1852(8), Patil, P., Kibiryeva, N., Uechi, T., Marshall, J., O'Brien, J.E., Artman, M., Kenmochi, N., Bittel, D.C., scaRNAs Regulate Splicing and Vertebrate Heart Development, 1619-29, Copyright (2015) with permission from Elsevier. Full text @ BBA Molecular Basis of Disease