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ZFIN ID: ZDB-FIG-120810-52
Hutchinson et al., 2012 - Tbl3 regulates cell cycle length during zebrafish development. Developmental Biology   368(2):261-272 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 3 tbl3MO phenocopies theceymutant. The tbl3 gene is 2694 base pairs and 25 exons long (A). Black boxes represent exons and lines joining boxes are introns. Lines with hash marks indicate introns of unknown length. Red lines above exons represent the location of the MO sequence used in this study. Blue lines represent WD repeats. Orange line represents UTP13 domain (A). SB MO is directed against the splice donor site at the 3′ end of exon 5 (A). Injection of MOs designed against the tbl3 gene results in a phenocopy of the cey mutant phenotype at 4 dpf. tbl3 morphant 4 dpf larvae have malformed jaws (black arrows), small eyes (white arrows; B, C) and a reduction of T cells labeled by p56lck (D, E) as compared to uninjected controls. HSPCs labeled by c-myb (F, I), intestinal cells labeled by ifabp (G, J), and exocrine pancreas labeled by trypsin (H, K) are all severely reduced as compared to controls and similar to cey mutants (Fig. 1). Images of larvae labeled with trypsin are pictures of the right side of the embryo reflected so that anterior is to the right (H, K, N). Injection of the tbl3MO into p53-/- mutants resulted in similar phenotypes (L–N) as compared to injection of tbl3MO into wild-type (I–K). Scale bars are 130μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fabp2 WT control Day 4 intestine ISH
WT + MO1-tbl3 standard conditions Day 4 intestine ISH
WT + MO2-tbl3 standard conditions Day 4 intestine ISH
tp53zy7/zy7 + MO1-tbl3 standard conditions Day 4 intestine ISH
tp53zy7/zy7 + MO2-tbl3 standard conditions Day 4 intestine ISH
lck cz1Tg control Day 4 T cell ISH
cz1Tg + MO1-tbl3 standard conditions Day 4 Not Detected T cell ISH
cz1Tg + MO2-tbl3 standard conditions Day 4 Not Detected T cell ISH
myb WT control Day 4 hematopoietic stem cell ISH
WT + MO1-tbl3 standard conditions Day 4 hematopoietic stem cell ISH
WT + MO2-tbl3 standard conditions Day 4 hematopoietic stem cell ISH
tp53zy7/zy7 + MO1-tbl3 standard conditions Day 4 hematopoietic stem cell ISH
tp53zy7/zy7 + MO2-tbl3 standard conditions Day 4 hematopoietic stem cell ISH
prss1 WT control Day 4 exocrine pancreas ISH
WT + MO1-tbl3 standard conditions Day 4 exocrine pancreas ISH
WT + MO2-tbl3 standard conditions Day 4 exocrine pancreas ISH
tp53zy7/zy7 + MO1-tbl3 standard conditions Day 4 exocrine pancreas ISH
tp53zy7/zy7 + MO2-tbl3 standard conditions Day 4 exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbl3 standard conditions Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 hematopoietic stem cell decreased amount, abnormal
Day 4 intestine hypoplastic, abnormal
WT + MO2-tbl3 standard conditions Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 hematopoietic stem cell decreased amount, abnormal
Day 4 intestine hypoplastic, abnormal
cz1Tg + MO1-tbl3 standard conditions Day 4 T cell absent, abnormal
cz1Tg + MO2-tbl3 standard conditions Day 4 T cell absent, abnormal
tp53zy7/zy7 + MO1-tbl3 standard conditions Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 hematopoietic stem cell decreased amount, abnormal
Day 4 intestine hypoplastic, abnormal
tp53zy7/zy7 + MO2-tbl3 standard conditions Day 4 exocrine pancreas hypoplastic, abnormal
tp53zy7/zy7 + MO2-tbl3 standard conditions Day 4 hematopoietic stem cell decreased amount, abnormal
tp53zy7/zy7 + MO2-tbl3 standard conditions Day 4 intestine hypoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 368(2), Hutchinson, S.A., Tooke-Locke, E., Wang, J., Tsai, S., Katz, T., and Trede, N.S., Tbl3 regulates cell cycle length during zebrafish development, 261-272, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.