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ZFIN ID: ZDB-FIG-120810-48
Hutchinson et al., 2012 - Tbl3 regulates cell cycle length during zebrafish development. Developmental Biology   368(2):261-272 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. S4 cey mutants have increased proliferation in the retina

Proliferation is expanded in cey mutant retinas. At 4 dpf there are more pH3+ (G2/M) cells in cey mutants (B) as compared to wild-type (A). In addition these cells are not confined to the peripheral CMZ region, but are throughout the central retina (white arrowheads; B). By 5 dpf the number of pH3+ cells is reduced and confined in cey larvae (D), more closely resembling wildtype larvae (C). Quantification shows that cey mutants have significantly more pH3+ cells than wild-type at 3, 4 and 5 dpf, although the number of cells decreases over time in both wild-type and cey mutants (E). Whole Z-stacks of dorsal view retinas were projected and the number of pH3+ cells were counted and then averaged to determine the average number of mitotic cells in wild-type and cey larvae at 3, 4 and 5 dpf (E, see methods for more details on quantification). cey mutants also have an increased number of cells in S-phase as compared to wild-type (F) indicating there are more proliferating cells at 3 dpf. The average number of BrdU positive cells in five lateral view sections was calculated for each retina (F, see methods for more details). Scale Bars are 130μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-h3 IHC WT standard conditions Day 4 ciliary marginal zone
IHC Day 5 ciliary marginal zone
IHC tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 ciliary marginal zone
IHC Day 4 retina central region
IHC Day 5 ciliary marginal zone
Phenotype Details
Fish Conditions Stage Phenotype
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Protruding-mouth cell population proliferation increased occurrence, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Protruding-mouth retina morphogenesis in camera-type eye process quality, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Protruding-mouth S phase increased duration, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Protruding-mouth - Day 5 M phase increased duration, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Protruding-mouth - Day 5 retina morphogenesis in camera-type eye process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 368(2), Hutchinson, S.A., Tooke-Locke, E., Wang, J., Tsai, S., Katz, T., and Trede, N.S., Tbl3 regulates cell cycle length during zebrafish development, 261-272, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.