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ZFIN ID: ZDB-FIG-120810-53
Hutchinson et al., 2012 - Tbl3 regulates cell cycle length during zebrafish development. Developmental Biology   368(2):261-272 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Day 4 to Day 6
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Day 4 to Day 6

Fig. 4 Loss of HSPCs differentially affects hematopoietic lineages.CD41::eGFPlow labels HSPCs (A, A′ white arrowheads) while CD41::eGFPhigh labels thrombocytes (A, A′ white asterisks) in the CHT of 4 dpf larvae. HSPCs (white arrowheads) are severely reduced in tbl3 morphants at 4 dpf (B, B2) as compared to uninjected wild-type controls (A, A′). In contrast, thrombocytes in tbl3 morphants are unaffected (A, A′, B, B′). There are 24.9+/- 10.2 thrombocytes in the CHT in wild-type (N=28 larvae), and 29+/11.8 in the CHT of cey (N=28) which is an insignificant difference (p-value=0.16). Brightfield images in (A) and (B) are the same larvae as (A2) and (B2), respectively. Dotted yellow boxes indicate area in CHT counted in G. Neutrophils labeled by mpx are unaffected in cey larvae (D) at 6 dpf as compared to wild-type (C), while red blood cells labeled by βE1-globin at 6 dpf are severely reduced in cey (E, F). The average number of HSPCs in the CHT of tbl3 morphants is normal at 2 dpf, but significantly reduced at 3 and 4 dpf (G). The average number of mitotic cells in the CHT of tbl3 morphant larvae is not significantly affected at 2 or 3 dpf, but is severely reduced at 4 dpf (H). The number of CD41::eGFPlow+/pH3+ cells is not significantly changed in the CHT of tbl3 morphant larvae at 3 or 4 dpf (I). The area of the CHT counted in (G–I) is labeled by the yellow dotted lines in (A), (A′), (B) and (B′). The error bars in (G) and (H) represent standard error of the mean. The error in (I) represents standard deviation. Scale bars are 130 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg control Day 4 hematopoietic stem cell IFL
Day 4 thrombocyte IFL
la2Tg + MO1-tbl3 standard conditions Day 4 hematopoietic stem cell IFL
Day 4 thrombocyte IFL
la2Tg + MO2-tbl3 standard conditions Day 4 hematopoietic stem cell IFL
Day 4 thrombocyte IFL
hbae1.1 WT standard conditions Day 6 nucleate erythrocyte ISH
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 6 nucleate erythrocyte ISH
mpx WT standard conditions Day 6 neutrophil ISH
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 6 neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg + MO1-tbl3 standard conditions Day 4 blood island has fewer parts of type hematopoietic stem cell, abnormal
la2Tg + MO1-tbl3 standard conditions Day 4 blood island has normal numbers of parts of type thrombocyte, normal
la2Tg + MO1-tbl3 standard conditions Day 4 hematopoietic stem cell proliferation decreased rate, abnormal
la2Tg + MO2-tbl3 standard conditions Day 4 blood island has fewer parts of type hematopoietic stem cell, abnormal
Day 4 blood island has normal numbers of parts of type thrombocyte, normal
Day 4 hematopoietic stem cell proliferation decreased rate, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 6 neutrophil normal amount, normal
Day 6 nucleate erythrocyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 368(2), Hutchinson, S.A., Tooke-Locke, E., Wang, J., Tsai, S., Katz, T., and Trede, N.S., Tbl3 regulates cell cycle length during zebrafish development, 261-272, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.