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ZFIN ID: ZDB-FIG-120810-50
Hutchinson et al., 2012 - Tbl3 regulates cell cycle length during zebrafish development. Developmental Biology   368(2):261-272 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 1 Many tissue types are severely reduced inceymutants. All figures are oriented anterior to the left. p56lck labels T cells in 4 dpf zebrafish larvae (A, yellow arrow). Cey mutants have severely reduced p56lck expression at 4 dpf (B, yellow arrow) suggesting a lack of T cells. In comparison to live wild-type larvae at 4 dpf (C), live cey mutants (D) have small eyes, heart edema (red arrow), and a deformed jaw (black arrow). Yellow dotted line designates the swim bladder (C, D). Although the tail is morphologically normal, there is a severe reduction of HSPCs in cey mutants at 4 dpf as shown by the severe reduction of c-myb positive cells in cey mutants (F) as compared to wild-type (E). Alcian blue staining indicates the mandibular and hyoid arches are smaller and malformed in cey mutants at 5 dpf (H) as compared to wild-type (G). Endodermal derivatives are severely reduced in cey mutants (I–L). Intestinal tissue labeled with ifabp is severely reduced in cey mutants at 4 dpf (I, J). In addition, exocrine pancreas labeled by trypsin is severely reduced in mutants at 5 dpf (K, L). The eyes of cey mutants are smaller than wild-type eyes at 5 dpf (M, N). Despite the smaller eye size, all cell layers are present in cey mutant retinas (O, P). Red asterisks label each retinal layer (O, P). Scale bars are 130 μm (A–L) and 95 μm (M–P). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fabp2 WT standard conditions Day 4 intestine ISH
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 intestine ISH
lck cz1Tg standard conditions Day 4 T cell ISH
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26; cz1Tg standard conditions Day 4 Not Detected T cell ISH
myb WT standard conditions Day 4 hematopoietic stem cell ISH
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 hematopoietic stem cell ISH
prss1 WT standard conditions Day 5 exocrine pancreas ISH
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 endoderm hypoplastic, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 eye decreased size, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 hematopoietic stem cell decreased amount, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 intestine decreased size, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 pericardium edematous, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 4 swim bladder hypoplastic, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 exocrine pancreas decreased size, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 eye decreased size, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 mandibular arch skeleton deformed, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 pharyngeal arch 1 decreased size, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 pharyngeal arch 1 malformed, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 pharyngeal arch 2 decreased size, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 pharyngeal arch 2 malformed, abnormal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26 standard conditions Day 5 retina layer formation process quality, normal
tbl3Df(Chr03:noxo1a,zgc:113100,sox8b,proza)cz26/cz26; cz1Tg standard conditions Day 4 T cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 368(2), Hutchinson, S.A., Tooke-Locke, E., Wang, J., Tsai, S., Katz, T., and Trede, N.S., Tbl3 regulates cell cycle length during zebrafish development, 261-272, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.