ZFIN ID: ZDB-FIG-111215-19
Nguyen et al., 2011 - A high level of liver-specific expression of oncogenic KrasV12 drives robust liver tumorigenesis in transgenic zebrafish. Disease models & mechanisms   4(6):801-13 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4 Hyperactivation of the MAPK signaling pathway in krasV12 transgenic zebrafish. (A) Determination of expression levels of various kinase genes by qRT-PCR in liver hyperplasia (3 mpf) and carcinoma (9 mpf). The expression levels of these genes in each WT and transgenic liver sample were first measured and normalized with the expression level of β-actin (n=5 each). The log2 fold changes in expression in the transgenic samples as compared with matched WT sample are presented. (B) Comparison of MAPK family expression during liver tumorigenesis in krasV12 transgenic zebrafish, human liver cancer and other experimental models of HCC. a(Schmidt et al., 1997); b(Wurmbach et al., 2007; Chang et al., 2009); c(Wurmbach et al., 2007). (C) Western blots of total proteins from WT normal liver (NWt) and kras V12 transgenic liver showing normal liver morphology (NTg), hyperplastic liver (H) or HCC (C) to detect total Kras (F234), Kras2B (C19) isoform, phospho-MEK1/2 and phospho-ERK1/2 (also known as phospho-p44/42 MAPK). Arrows: double bands of phospho-ERK1/2 (44 kDa and 42 kDa). β-actin, internal control for equal loading. (D) Immunohistochemical analysis of paraffin-embedded liver sections from WT (control; top row), 3-mpf transgenic (hyperplasia; middle row) and 9-mpf transgenic (carcinoma; bottom row) fish. Sections were stained with antibodies against phospho-MEK1/2 or phospho-ERK1/2. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
braf gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
map2k1 gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
map2k2a gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
map4k2 gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
map4k5 gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
mapk1 gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
mapk3 gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
mapk8b gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
mapk12a gz21Tg standard conditions Adult liver hyperplastic tissue RTPCR
mapk14a gz21Tg standard conditions Adult liver hyperplastic tissue RTPCR
mapkapk5 gz21Tg standard conditions Adult liver hyperplastic tissue RTPCR
raf1a gz21Tg standard conditions Adult liver hepatocellular carcinoma RTPCR
Adult liver hyperplastic tissue RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-kras WB WT standard conditions Adult liver
WB gz21Tg standard conditions Adult liver
WB Adult liver hepatocellular carcinoma
WB Adult liver hyperplastic tissue
Ab2-kras WB WT standard conditions Adult Not Detected liver
WB gz21Tg standard conditions Adult liver
WB Adult liver hepatocellular carcinoma
WB Adult liver hyperplastic tissue
Phenotype Details
Fish Conditions Stage Phenotype
gz21Tg standard conditions Adult ERK1 and ERK2 cascade increased occurrence, abnormal
gz21Tg standard conditions Adult JNK cascade increased occurrence, abnormal
gz21Tg standard conditions Adult liver hyperplastic, abnormal
gz21Tg standard conditions Adult liver hepatocellular carcinoma present, abnormal
gz21Tg standard conditions Adult p38MAPK cascade increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.