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ZFIN ID: ZDB-FIG-090602-16
Arduini et al., 2009 - Genetic ablation of neural crest cell diversification. Development (Cambridge, England)   136(12):1987-1994 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 2 Failure of NC sublineage specification in foxd3zdf10;tfap2alow double mutants. (A-D) Lateral views with anterior to the left. (A) mitf expression at 24 hpf (arrowheads). foxd3zdf10;tfap2alow double mutant embryos completely lack NC-derived melanoblast mitf expression. (B) xanthine dehydrogenase (xdh), diagnostic of xanthophore precursors, at 30 hpf. (C) endothelin receptor B (ednrb1), presumed to be expressed by all chromatophore precursors, at 30 hpf and (D) leukocyte tyrosine kinase (ltk), reported to be expressed by iridophore precursors, at 30 hpf. (E) Dorsal views of dhand expression, anterior to left, ventral of the caudal hindbrain, at 48 hpf. Sympathetic neuron precursors are labeled in wild-type embryos (arrowheads), as are the more ventral and laterally localized enteric neuron precursors (arrows). In the majority of foxd3zdf10 embryos, both sympathetic and enteric neuron progenitors are absent (asterisk) (Stewart et al., 2006). In tfap2alow mutants, sympathetic neuron precursors are absent whereas a small number of enteric precursors are present (arrow) (Knight et al., 2003). In foxd3zdf10;tfap2alow double mutants, both sympathetic and enteric neuron precursors are absent (asterisk). (F-J) Lateral (F-I) and dorsolateral views (J), anterior to the left. (F) dlx2 expression at 24 hpf. dlx2 expression (arrowheads) is absent in the branchial arches of double mutant embryos, but is retained in the forebrain (arrows). (G) dlx3 expression at 30 hpf; (H) dhand expression at 30 hpf. NC expression of both genes is absent in double mutant embryos with dlx3 expression maintained in non-NC otic vesicle (asterisks). (I) tbx1 expression in the branchial arches (asterisk) at 30 hpf is diagnostic of the endodermal component of the arches. tbx1 expression is normal in all experimental embryos. (J) endothelin 1 (edn1) expression in the mesodermal component of the branchial arches at 30 hpf. Branchial mesoderm appears to develop normally in foxd3zdf10 and tfap2alow single mutants, as well as in foxd3zdf10;tfap2alow double mutant embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aox5 WT standard conditions Prim-15 xanthoblast ISH
foxd3zdf10/zdf10 standard conditions Prim-15 xanthoblast ISH
tfap2ats213/ts213 standard conditions Prim-15 xanthoblast ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-15 Not Detected xanthoblast ISH
dlx2a WT standard conditions Prim-5 forebrain ISH
Prim-5 pharyngeal arch 3-7 ISH
foxd3zdf10/zdf10 standard conditions Prim-5 forebrain ISH
Prim-5 pharyngeal arch 3-7 ISH
tfap2ats213/ts213 standard conditions Prim-5 forebrain ISH
Prim-5 pharyngeal arch 3-7 ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-5 forebrain ISH
Prim-5 Not Detected pharyngeal arch 3-7 ISH
dlx3b WT standard conditions Prim-15 otic vesicle ISH
Prim-15 pharyngeal arch 3-7 ISH
foxd3zdf10/zdf10 standard conditions Prim-15 otic vesicle ISH
Prim-15 pharyngeal arch 3-7 ISH
tfap2ats213/ts213 standard conditions Prim-15 otic vesicle ISH
Prim-15 pharyngeal arch 3-7 ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-15 otic vesicle ISH
edn1 WT standard conditions Prim-15 pharyngeal arch 3-7 mesoderm ISH
foxd3zdf10/zdf10 standard conditions Prim-15 pharyngeal arch 3-7 mesoderm ISH
tfap2ats213/ts213 standard conditions Prim-15 pharyngeal arch 3-7 mesoderm ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-15 pharyngeal arch 3-7 mesoderm ISH
ednrba WT standard conditions Prim-15 pigment cell ISH
foxd3zdf10/zdf10 standard conditions Prim-15 pigment cell ISH
tfap2ats213/ts213 standard conditions Prim-15 pigment cell ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-15 Not Detected pigment cell ISH
hand2 WT standard conditions Prim-15 pharyngeal arch 3-7 ISH
Long-pec enteric nervous system ISH
Long-pec sympathetic nervous system ISH
foxd3zdf10/zdf10 standard conditions Prim-15 pharyngeal arch 3-7 ISH
Long-pec Not Detected enteric nervous system ISH
Long-pec Not Detected sympathetic nervous system ISH
tfap2ats213/ts213 standard conditions Prim-15 pharyngeal arch 3-7 ISH
Long-pec enteric nervous system ISH
Long-pec Not Detected sympathetic nervous system ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-15 Not Detected pharyngeal arch 3-7 ISH
Long-pec Not Detected enteric nervous system ISH
Long-pec Not Detected sympathetic nervous system ISH
ltk WT standard conditions Prim-15 iridoblast ISH
foxd3zdf10/zdf10 standard conditions Prim-15 Not Detected iridoblast ISH
tfap2ats213/ts213 standard conditions Prim-15 Not Detected iridoblast ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-15 Not Detected iridoblast ISH
mitfa WT standard conditions Prim-5 melanoblast ISH
foxd3zdf10/zdf10 standard conditions Prim-5 melanoblast ISH
tfap2ats213/ts213 standard conditions Prim-5 melanoblast ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-5 Not Detected melanoblast ISH
tbx1 WT standard conditions Prim-15 pharyngeal arch 3-7 ISH
foxd3zdf10/zdf10 standard conditions Prim-15 pharyngeal arch 3-7 ISH
tfap2ats213/ts213 standard conditions Prim-15 pharyngeal arch 3-7 ISH
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-15 pharyngeal arch 3-7 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tfap2ats213/ts213; foxd3zdf10/zdf10 standard conditions Prim-5 neural crest cell development disrupted, abnormal
Prim-5 pigment cell differentiation disrupted, abnormal
Prim-15 neural crest cell development disrupted, abnormal
Prim-15 pigment cell differentiation disrupted, abnormal
Long-pec neural crest cell development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development