PUBLICATION

Genetic ablation of neural crest cell diversification

Authors
Arduini, B.L., Bosse, K.M., and Henion, P.D.
ID
ZDB-PUB-090518-13
Date
2009
Source
Development (Cambridge, England)   136(12): 1987-1994 (Journal)
Registered Authors
Arduini, Brigitte, Bosse, Kevin, Henion, Paul
Keywords
none
MeSH Terms
  • Animals
  • Cell Lineage/physiology*
  • Embryo, Nonmammalian/physiology
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Mutation
  • Neural Crest/embryology
  • Neural Crest/physiology*
  • SOX9 Transcription Factor/genetics
  • SOX9 Transcription Factor/metabolism
  • Transcription Factor AP-2/genetics
  • Transcription Factor AP-2/metabolism
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
19439494 Full text @ Development
Abstract
The neural crest generates multiple cell types during embryogenesis but the mechanisms regulating neural crest cell diversification are incompletely understood. Previous studies using mutant zebrafish indicated that foxd3 and tfap2a function early and differentially in the development of neural crest sublineages. Here, we show that the simultaneous loss of foxd3 and tfap2a function in zebrafish foxd3(zdf10);tfap2a(low) double mutant embryos globally prevents the specification of developmentally distinct neural crest sublineages. By contrast, neural crest induction occurs independently of foxd3 and tfap2a function. We show that the failure of neural crest cell diversification in double mutants is accompanied by the absence of neural crest sox10 and sox9a/b gene expression, and that forced expression of sox10 and sox9a/b differentially rescues neural crest sublineage specification and derivative differentiation. These results demonstrate the functional necessity for foxd3 and tfap2a for neural crest sublineage specification and that this requirement is mediated by the synergistic regulation of the expression of SoxE family genes. Our results identify a genetic regulatory pathway functionally discrete from the process of neural crest induction that is required for the initiation of neural crest cell diversification during embryonic development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping