ZFIN ID: ZDB-FIG-080715-39
Dutta et al., 2008 - Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary. Developmental Biology   319(2):248-257 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: cga, gh1, pomca, prl, tshba
Fish: kca4Tg, kca3Tg; kca4Tg
Condition: heat shock
Anatomical Terms: adenohypophysis, hypophysis, neurohypophysis, pars intermedia
Stage: Protruding-mouth

Fig. 5 Gain of Notch function increases the number of pomc expressing corticotropes and melanotropes at the expense of lactotropes and thyrotropes. Embryos carrying either the hsp70:Gal4 or the hsp70:Gal4 x UAS:notch1a transgenes were heat shocked at Bud (10 h) stage and analyzed for hormone expression at Protruding mouth (72 h) stage (A–J). In heat-shocked hsp70:Gal4 x UAS:notch1a transgenic embryos, both prl (F, n = 36/36) and tsh-β (I, n = 42/42) are completely lost, pomc expression is expanded in APD, PPD and PI (G, n = 32/32), and cga is expanded (J, n = 15/17). gh expression is unaffected (H, n = 40/40) compared to prl (A, n = 34) and tsh-β (D, n = 32), pomc (B, n = 37), gh (C, n = 30) and cga (E, n = 20) in hsp70:Gal4 embryos following heat shock. Dotted lines indicate outline of anterior pituitary tissue (omitted in panels where pituitary border was ambiguous). (A–J) Ventral views anterior to the top. Scale bar: 15 μm (A–J).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cga kca3Tg; kca4Tg heat shock Protruding-mouth hypophysis ISH
kca4Tg heat shock Protruding-mouth hypophysis ISH
gh1 kca3Tg; kca4Tg heat shock Protruding-mouth hypophysis ISH
kca4Tg heat shock Protruding-mouth hypophysis ISH
pomca kca3Tg; kca4Tg heat shock Protruding-mouth adenohypophysis ISH
Protruding-mouth neurohypophysis ISH
Protruding-mouth pars intermedia ISH
kca4Tg heat shock Protruding-mouth adenohypophysis ISH
Protruding-mouth neurohypophysis ISH
Protruding-mouth pars intermedia ISH
prl kca3Tg; kca4Tg heat shock Protruding-mouth Not Detected hypophysis ISH
kca4Tg heat shock Protruding-mouth hypophysis ISH
tshba kca3Tg; kca4Tg heat shock Protruding-mouth Not Detected hypophysis ISH
kca4Tg heat shock Protruding-mouth hypophysis ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Dutta, S., Dietrich, J.E., Westerfield, M., and Varga, Z.M., Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary, 248-257, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.