ZFIN ID: ZDB-FIG-080715-35
Dutta et al., 2008 - Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary. Developmental Biology   319(2):248-257 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: ctslb, dla, notch1a, pitx3
Fish: WT
Anatomical Terms: adenohypophyseal placode, head, polster
Stage Range: Bud to 10-13 somites

Fig. 1 Prospective pituitary precursors express deltaA and notch1a after the 10-somite (14 h) stage. Wild-type embryos doubly labeled with mRNA probes for cathepsin L, 1b (ctsl1b), previously known as hatching gland gene 1 (hgg1; A–F, red) and pitx3 (A, B), deltaA (C, D), or notch1a (E, F). (A, C, E) At Bud stage, presumptive pituitary precursor cells express pitx3 (A, n = 25; dotted lines indicate approximate location according to Dutta et al., 2005). deltaA (C, n = 30) and notch1a (E, n = 30) are not expressed in pituitary precursors. At 10-somite stage (14 h), anterior cranial placode precursors (arrowheads) express pitx3 (B, n = 25), deltaA (D, n = 25), and notch1a (F, n = 25). (A, C, E) Dorsal views, anterior to the top (dotted lines indicate approximate location of pituitary precursor cells according to Dutta et al., 2005); (B, D, F) dorsal views of prospective head region, anterior to the top. Scale bar: 50 mμm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ctslb WT standard conditions Bud polster ISH
10-13 somites head ISH
dla WT standard conditions Bud Not Detected adenohypophyseal placode ISH
10-13 somites head ISH
notch1a WT standard conditions Bud Not Detected adenohypophyseal placode ISH
10-13 somites head ISH
pitx3 WT standard conditions Bud adenohypophyseal placode ISH
10-13 somites head ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Dutta, S., Dietrich, J.E., Westerfield, M., and Varga, Z.M., Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary, 248-257, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.