ZFIN ID: ZDB-FIG-080715-37
Dutta et al., 2008 - Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary. Developmental Biology   319(2):248-257 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: lhx3, prl
Fish: WT, mib1ta52b/ta52b, kca3Tg; kca4Tg
Conditions: chemical treatment: DAPT, heat shock
Anatomical Term: adenohypophyseal placode
Stage: Prim-5

Fig. 3 Notch is necessary and sufficient to restrict lactotrope differentiation in the anterior pituitary placode. (A, B) Wild-type embryos labeled with lim3 (A, n = 85) and prl (B, n = 96). (B, inset) Bilateral cells in the anterior pituitary placode express prl. Gene expression in DMSO treated (n = 30), and hsp70:Gal4 heat-shocked (n = 30) control embryos was similar to wild-type embryos in A and B. (A, C, E, G) Pituitary placode size and lim3 expression in mib mutant (C, n = 39), in DAPT treated (E, n = 45), and in heat-shocked hsp70:Gal4 × UAS:notch1a (G, n = 22) transgenic embryos is similar to controls (A, B). (B, D, F) Loss of functional Notch signaling in mib mutant (D, inset, n = 37/37) and DAPT treated (F, inset, n = 32/40) embryos lead to expansion of prl in pituitary placode (arrowheads; D, F). (H) prl expression in the anterior pituitary placode is completely lost in hsp70:Gal4 x UAS:notch1a transgenic embryos (n = 48/48) following heat shock. (A–G) side views, anterior to the left, dorsal to the top; (A–G, inset) frontal views, dorsal to the top. All embryos Prim-5 (24 h) stage. Scale bar: 50 μm (A–H); Insets A, C, E, G: 40 μm; Insets B, D, F, H: 30 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lhx3 WT standard conditions Prim-5 adenohypophyseal placode ISH
WT chemical treatment: DAPT Prim-5 adenohypophyseal placode ISH
kca3Tg; kca4Tg heat shock Prim-5 adenohypophyseal placode ISH
mib1ta52b/ta52b standard conditions Prim-5 adenohypophyseal placode ISH
prl WT standard conditions Prim-5 adenohypophyseal placode ISH
WT chemical treatment: DAPT Prim-5 adenohypophyseal placode ISH
kca3Tg; kca4Tg heat shock Prim-5 Not Detected adenohypophyseal placode ISH
mib1ta52b/ta52b standard conditions Prim-5 adenohypophyseal placode ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Dutta, S., Dietrich, J.E., Westerfield, M., and Varga, Z.M., Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary, 248-257, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.