ZFIN ID: ZDB-FIG-080715-38
Dutta et al., 2008 - Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary. Developmental Biology   319(2):248-257 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: cga, gh1, pomca, prl, tshba
Fish: WT, mib1ta52b/ta52b
Condition: chemical treatment: DAPT
Anatomical Terms: adenohypophysis, hypophysis, neurohypophysis, pars intermedia
Stage: Protruding-mouth

Fig. 4 Notch signaling is necessary for corticotrope and somatotrope differentiation. (A–E) Wild-type (Gene expression in DMSO treated control embryos, not shown, was same as in wild-type embryos in panels A–E), mib mutant (F–J) and DAPT treated embryos (K–O) labeled with probes for anterior pituitary hormones at 72 h. In mib mutant embryos, prl (F, n = 25/25), tsh-β (I, n = 23/23) and cga (J, n = 25/25) in the anterior pituitary is expanded, pomc expression is lost in APD and reduced in PPD (G, n = 26/26), and gh expression (H, n = 52/52) is completely lost compared to prl (A, n = 37), tsh-β (D, n = 40) and cga (E, n = 40), pomc (B, n = 20), gh expression (C, n = 40) in siblings. DAPT treatment between shield stage and 72 h leads to expanded prl (K, n = 26/33), tsh-β (n = 50/60) and cga expression (O, n = 35/42), whereas pomc expression in APD (L, n = 23/25) is lost or severely reduced, pomc expression in PI (L, n = 22/25) is somewhat reduced and gh expression (M, n = 35/40) is entirely absent in the PPD compared to wild-type embryos (A–E). Dotted lines indicate outline of anterior pituitary tissue (omitted in panels where pituitary border was ambiguous). Arrowhead shows pomc expression in PI. (A–O) Ventral views, anterior to the top. Scale bar: 25 μm (A–O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cga WT standard conditions Protruding-mouth adenohypophysis ISH
Protruding-mouth hypophysis ISH
WT chemical treatment: DAPT Protruding-mouth adenohypophysis ISH
mib1ta52b/ta52b standard conditions Protruding-mouth adenohypophysis ISH
gh1 WT standard conditions Protruding-mouth adenohypophysis ISH
Protruding-mouth hypophysis ISH
WT chemical treatment: DAPT Protruding-mouth Not Detected adenohypophysis ISH
Protruding-mouth Not Detected hypophysis ISH
mib1ta52b/ta52b standard conditions Protruding-mouth Not Detected hypophysis ISH
pomca WT standard conditions Protruding-mouth adenohypophysis ISH
Protruding-mouth hypophysis ISH
Protruding-mouth pars intermedia ISH
WT chemical treatment: DAPT Protruding-mouth adenohypophysis ISH
mib1ta52b/ta52b standard conditions Protruding-mouth Not Detected adenohypophysis ISH
Protruding-mouth neurohypophysis ISH
prl WT standard conditions Protruding-mouth adenohypophysis ISH
Protruding-mouth hypophysis ISH
WT chemical treatment: DAPT Protruding-mouth adenohypophysis ISH
mib1ta52b/ta52b standard conditions Protruding-mouth adenohypophysis ISH
tshba WT standard conditions Protruding-mouth adenohypophysis ISH
Protruding-mouth hypophysis ISH
WT chemical treatment: DAPT Protruding-mouth adenohypophysis ISH
mib1ta52b/ta52b standard conditions Protruding-mouth adenohypophysis ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Dutta, S., Dietrich, J.E., Westerfield, M., and Varga, Z.M., Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary, 248-257, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.