ZFIN ID: ZDB-FIG-080715-36
Dutta et al., 2008 - Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary. Developmental Biology   319(2):248-257 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: dla, dlb, dld, notch1a, pitx3 (all 6) expand
Fish: WT
Anatomical Term: adenohypophyseal placode
Stage Range: 20-25 somites to Prim-5

Fig. 2 At the 20-somite (19 h) stage, pituitary placode cells express deltaA, deltaB, and deltaD in a salt and pepper pattern. Wild-type embryos are labeled with probes for deltaA (A, E, n = 19), deltaB (B, F, n = 28), and deltaD (C, G, n = 25). (D) Expression of notch1a (blue, n = 20) and prl (red, n = 20) does not colocalize in the pituitary placode at prim-5 stage (24 h). (H) Cells in the pituitary placode co-express pitx3 (red, n = 20) and deltaA (blue, n = 20). (A–H) Arrowheads indicate cells in the pituitary placode, asterisk indicates optic recess. (A–C) Side views, anterior to the left, dorsal to the top; (D–H) frontal views, dorsal to the top. Scale bar: 50 μm (A–C, E–G), 20 μm (D, H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dla WT standard conditions 20-25 somites adenohypophyseal placode ISH
Prim-5 adenohypophyseal placode ISH
dlb WT standard conditions 20-25 somites adenohypophyseal placode ISH
dld WT standard conditions 20-25 somites adenohypophyseal placode ISH
notch1a WT standard conditions Prim-5 adenohypophyseal placode ISH
pitx3 WT standard conditions Prim-5 adenohypophyseal placode ISH
prl WT standard conditions Prim-5 adenohypophyseal placode ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Dutta, S., Dietrich, J.E., Westerfield, M., and Varga, Z.M., Notch signaling regulates endocrine cell specification in the zebrafish anterior pituitary, 248-257, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.