Fig. 7
- ID
- ZDB-FIG-250204-43
- Publication
- Sun et al., 2024 - Target protein identification in live cells and organisms with a non-diffusive proximity tagging system
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POST-IT enables in vivo target-ID. (A) Schematic of experimental design for applying POST-IT in zebrafish. A plasmid construct containing the POST-IT system was injected into embryos at the one-cell stage. The injected embryos were dechorionated 24 hr later, and then treated with DH5 or DMSO for an additional 24 hr. Embryos lysates were collected and analyzed by immunoblot. (B) Representative images of injected embryos. Bright-field (BF) images reveal no overt toxicity from POST-IT expression. EGFP images display robust expression of POST-IT. Scale bar, 500 µm. (C) Immunoblot analysis demonstrates that POST-IT exhibits significant enrichment of SRC in the presence of DH5 but not DMSO. |