Fig. 2 - Supplemental 3

Halo8KR-PafA demonstrates efficient labeling with HaloTag ligands. (A) Fluorescence polarization (FP) assay using 2 nM of HaloTag Alexa Fluor-488 (Halo-AF488) at 25°C. n = 2. Data are shown as mean ± s.d. (B) Western blot analysis for the Halo-Biotin competition assay. Halo-Biotin (5 μM) was incubated with 1 μM of Myc-Halo8KR-PafA at 37°C for 1 hr after a preincubation with 2 μM of DH1 for the indicated time period. Levels of bound Halo-Biotin were assessed by streptavidin-HRP. (C) Quantitative analysis of western blot results shown in (B). n = 5. Data are shown as mean ± s.e.m. p-Values were calculated by an unpaired two-sided t-test. **p<0.01, ***p<0.001; versus no DH1 competition.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife