Fig. 3 - Supplemental 2

Identification of self-pupylated lysine residues in PafA. (A) Mass spectrometry analysis identified K172 as the most frequently pupylated lysine in PafA. An inset provides an enlarged view of the Nano-LC-MS/MS mass spectrum. (B) Constructs of Halo-PafA and Halo8KR-PafAS126A,K172R containing 8KR mutations in HaloTag and mutations of S126A and K172R in PafA.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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