ZFIN ID: ZDB-FIG-200406-153
Zhao et al., 2020 - Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis. Cell communication and signaling : CCS   18:45 Full text @ Cell Commun. Signal.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 4

Fig. 7

Retina development in atp7a−/− mutant treated with copper. A TEM analysis of retinal cells in copper-stressed atp7a−/− mutant embryos at 96 hpf. A1-A3, sagittal slides of retina, red color indicating mitochondria and green color indicating ER. B Quantitative PCR of ROS related genes (B1; cox4i2, hmox2, lox, and nrf2), UPR related genes (B2; ire1a, perk, and atf6), and retinal genes (B3; opn1wl1, opn1sw1, opn1sw2 and rhodopsin) in atp7a−/− mutants. C WISH assays of ER stress marker bip and chop in atp7a−/− mutants. D WISH assays of retinal marker (opn1wl1 and opn1sw1) in atp7a−/− mutants. C1-C8, lateral view, anterior to the left, D1-D8, dorsal view, anterior to the up. Scale bar: A1-A3, 1 μm; C1-C8 and D1-D4, 100 μm; **, P < 0.01; *, P < 0.05

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atf6 AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
cox4i2 AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
ddit3 AB control Day 4 brain ISH
atp7ahza4/hza4 control Day 4 brain ISH
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 brain ISH
atp7ahza4/hza4 chemical treatment: copper atom Day 4 brain ISH
eif2ak3 AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
ern1 AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
hmox2a AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
hspa5 AB control Day 4 brain ISH
atp7ahza4/hza4 control Day 4 brain ISH
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 brain ISH
atp7ahza4/hza4 chemical treatment: copper atom Day 4 brain ISH
loxa AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
opn1lw1 AB control Day 4 retina ISH
Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 retina ISH
Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 retina ISH
Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 retina ISH
Day 4 whole organism RTPCR
opn1sw1 AB control Day 4 retina ISH
Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 retina ISH
Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 retina ISH
Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 retina ISH
Day 4 whole organism RTPCR
opn1sw2 AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
rho AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
znrf2a AB control Day 4 whole organism RTPCR
atp7ahza4/hza4 control Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 whole organism RTPCR
atp7ahza4/hza4 chemical treatment: copper atom Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
atp7ahza4/hza4 control Day 4 whole organism atf6 expression increased amount, abnormal
Day 4 whole organism cox4i2 expression increased amount, abnormal
Day 4 whole organism eif2ak3 expression increased amount, abnormal
Day 4 whole organism ern1 expression increased amount, abnormal
Day 4 whole organism hmox2a expression increased amount, abnormal
Day 4 whole organism loxa expression increased amount, abnormal
Day 4 whole organism znrf2a expression increased amount, abnormal
atp7ahza4/hza4 standard conditions Day 4 retina mitochondrion swollen, abnormal
atp7ahza4/hza4 chemical treatment: copper(2+) Day 4 brain ddit3 expression decreased amount, abnormal
Day 4 brain hspa5 expression decreased amount, abnormal
Day 4 retina opn1sw1 expression decreased amount, abnormal
Day 4 retina mitochondrion swollen, abnormal
Day 4 whole organism atf6 expression amount, ameliorated
Day 4 whole organism cox4i2 expression amount, ameliorated
Day 4 whole organism eif2ak3 expression amount, ameliorated
Day 4 whole organism ern1 expression amount, ameliorated
Day 4 whole organism hmox2a expression amount, ameliorated
Day 4 whole organism loxa expression amount, ameliorated
Day 4 whole organism opn1sw1 expression decreased amount, abnormal
Day 4 whole organism opn1sw2 expression decreased amount, abnormal
Day 4 whole organism rho expression decreased amount, abnormal
Day 4 whole organism znrf2a expression amount, ameliorated
atp7ahza4/hza4 chemical treatment: copper atom Day 4 brain ddit3 expression decreased amount, abnormal
Day 4 brain hspa5 expression decreased amount, abnormal
Day 4 retina opn1sw1 expression decreased amount, abnormal
Day 4 retina mitochondrion swollen, abnormal
Day 4 whole organism atf6 expression amount, ameliorated
Day 4 whole organism cox4i2 expression amount, ameliorated
Day 4 whole organism eif2ak3 expression amount, ameliorated
Day 4 whole organism ern1 expression amount, ameliorated
Day 4 whole organism hmox2a expression amount, ameliorated
Day 4 whole organism loxa expression amount, ameliorated
Day 4 whole organism opn1sw1 expression decreased amount, abnormal
Day 4 whole organism opn1sw2 expression decreased amount, abnormal
Day 4 whole organism rho expression decreased amount, abnormal
Day 4 whole organism znrf2a expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell communication and signaling : CCS for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Commun. Signal.