ZFIN ID: ZDB-FIG-200406-152
Zhao et al., 2020 - Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis. Cell communication and signaling : CCS   18:45 Full text @ Cell Commun. Signal.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 4

Fig. 6

Retina development in cox17−/− mutant treated with copper. A TEM analysis of retinal cells in copper stressed cox17−/− mutant embryos at 96 hpf. A1-A3, sagittal slides of retina, red color indicating mitochondria and green color indicating ER. B Quantitative PCR of ROS related genes (B1; cox4i2, hmox2, lox, and nrf2), UPR related genes (B2; ire1a, perk, and atf6), and retinal genes (B3; opn1wl1, opn1sw1, opn1sw2 and rhodopsin) in cox17−/− mutants. C WISH assays of ER stress marker bip and chop in cox17−/− mutants. D WISH assays of retinal marker genes (opn1wl1 and opn1sw1) in cox17−/− mutants. C1-C8, lateral view, anterior to the left, D1-D8, dorsal view, anterior to the up, and Scale bar: A1-A3, 1 μm; C1-C8 and D1-D8, 100 μm. **, P < 0.01; *, P < 0.05

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atf6 AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
cox4i2 AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
ddit3 AB control Day 4 brain ISH
cox17hza3/hza3 control Day 4 brain ISH
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 brain ISH
cox17hza3/hza3 chemical treatment: copper atom Day 4 brain ISH
eif2ak3 AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
ern1 AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
hmox2a AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
hspa5 AB control Day 4 brain ISH
cox17hza3/hza3 control Day 4 brain ISH
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 brain ISH
cox17hza3/hza3 chemical treatment: copper atom Day 4 brain ISH
loxa AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
opn1lw1 AB control Day 4 retina ISH
Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 retina ISH
Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 retina ISH
Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 retina ISH
Day 4 whole organism RTPCR
opn1sw1 AB control Day 4 retina ISH
Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 retina ISH
Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 retina ISH
Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 retina ISH
Day 4 whole organism RTPCR
opn1sw2 AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
rho AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
znrf2a AB control Day 4 whole organism RTPCR
cox17hza3/hza3 control Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 whole organism RTPCR
cox17hza3/hza3 chemical treatment: copper atom Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cox17hza3/hza3 control Day 4 retina opn1lw1 expression decreased amount, abnormal
Day 4 retina opn1sw1 expression decreased amount, abnormal
Day 4 whole organism opn1lw1 expression decreased amount, abnormal
Day 4 whole organism opn1sw1 expression decreased amount, abnormal
Day 4 whole organism rho expression decreased amount, abnormal
cox17hza3/hza3 standard conditions Day 4 retina mitochondrion morphology, normal
cox17hza3/hza3 chemical treatment: copper(2+) Day 4 retina opn1lw1 expression decreased amount, abnormal
Day 4 retina opn1sw1 expression decreased amount, abnormal
Day 4 retina mitochondrion swollen, abnormal
Day 4 whole organism atf6 expression increased amount, abnormal
Day 4 whole organism cox4i2 expression decreased amount, abnormal
Day 4 whole organism eif2ak3 expression increased amount, abnormal
Day 4 whole organism ern1 expression increased amount, abnormal
Day 4 whole organism loxa expression decreased amount, abnormal
Day 4 whole organism opn1lw1 expression decreased amount, abnormal
Day 4 whole organism opn1sw1 expression decreased amount, abnormal
Day 4 whole organism rho expression decreased amount, abnormal
Day 4 whole organism znrf2a expression decreased amount, abnormal
cox17hza3/hza3 chemical treatment: copper atom Day 4 retina opn1lw1 expression decreased amount, abnormal
Day 4 retina opn1sw1 expression decreased amount, abnormal
Day 4 retina mitochondrion swollen, abnormal
Day 4 whole organism atf6 expression increased amount, abnormal
Day 4 whole organism cox4i2 expression decreased amount, abnormal
Day 4 whole organism eif2ak3 expression increased amount, abnormal
Day 4 whole organism ern1 expression increased amount, abnormal
Day 4 whole organism loxa expression decreased amount, abnormal
Day 4 whole organism opn1lw1 expression decreased amount, abnormal
Day 4 whole organism opn1sw1 expression decreased amount, abnormal
Day 4 whole organism rho expression decreased amount, abnormal
Day 4 whole organism znrf2a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell communication and signaling : CCS for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Commun. Signal.