ZFIN ID: ZDB-FIG-200406-150
Zhao et al., 2020 - Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis. Cell communication and signaling : CCS   18:45 Full text @ Cell Commun. Signal.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 4

Fig. 5

Rescue of retinal defects with the addition of ROS scavenger and ER stress scavenger. ROS scavengers GSH & NAC and ER scavenger PBA recovered the copper induced small eye defects (A) and the expression of retinal markers opn1sw2 (B1-B5) & opn1lw1 (C1-C5 and D1-D5) in copper-stressed embryos. E ROS scavengers GSH significantly neutralized copper induced cell apoptosis in embryos. Average number of apoptotic cells of retinal sections in each group (E10) (n > 3, 3–5 sections from each embryo were used for counting the red positive apoptotic cells). B1-B5, C1-C5, and D1-D5, dorsal view, anterior to the up; E1- E9, sagittal slides in eyes domain. Scale bar, A, B1-B5, C1-C5, and D1-D5, 100 μm; E1-E9, 50 μm. **, P < 0.01; *, P < 0.05

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
opn1lw1 AB control Day 4 retina ISH
AB chemical treatment: copper(2+) Day 4 retina ISH
AB chemical treatment: N-acetyl-L-cysteine, chemical treatment: copper(2+) Day 4 retina ISH
AB chemical treatment: 4-phenylbutyric acid, chemical treatment: copper(2+) Day 4 retina ISH
AB chemical treatment: copper atom Day 4 retina ISH
AB chemical treatment: N-acetyl-L-cysteine, chemical treatment: copper atom Day 4 retina ISH
AB chemical treatment: 4-phenylbutyric acid, chemical treatment: copper atom Day 4 retina ISH
opn1sw2 AB control Day 4 retina ISH
AB chemical treatment: copper(2+) Day 4 retina ISH
AB chemical treatment: copper(2+), chemical treatment: glutathione Day 4 retina ISH
AB chemical treatment: copper atom Day 4 retina ISH
AB chemical treatment: glutathione, chemical treatment: copper atom Day 4 retina ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment: copper(2+) Day 4 retina opn1lw1 expression decreased amount, abnormal
Day 4 retina opn1sw2 expression decreased amount, abnormal
AB chemical treatment: copper(2+), chemical treatment: glutathione Day 4 retina opn1sw2 expression amount, ameliorated
AB chemical treatment: N-acetyl-L-cysteine, chemical treatment: copper(2+) Day 4 retina opn1lw1 expression amount, ameliorated
AB chemical treatment: 4-phenylbutyric acid, chemical treatment: copper(2+) Day 4 retina opn1lw1 expression amount, ameliorated
AB chemical treatment: copper atom Day 4 eye decreased diameter, abnormal
Day 4 retina opn1lw1 expression decreased amount, abnormal
Day 4 retina opn1sw2 expression decreased amount, abnormal
AB chemical treatment: glutathione, chemical treatment: copper atom Day 4 eye diameter, ameliorated
Day 4 retina opn1sw2 expression amount, ameliorated
AB chemical treatment: N-acetyl-L-cysteine, chemical treatment: copper atom Day 4 eye diameter, ameliorated
Day 4 retina opn1lw1 expression amount, ameliorated
AB chemical treatment: 4-phenylbutyric acid, chemical treatment: copper atom Day 4 eye diameter, ameliorated
Day 4 retina opn1lw1 expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell communication and signaling : CCS for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Commun. Signal.