FIGURE

Fig. 4

ID

ZDB-FIG-200101-48

Publication

Li et al., 2019 - SOX19b regulates the premature neuronal differentiation of neural stem cells through EZH2-mediated histone methylation in neural tube development of zebrafish

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Fig. 4

NSCs in embryos injected with sox19b MO were prematurely differentiated. a Quantitative RT-PCR analysis for Ngn1, ascl1a, HuC, lslet1, and her3. Compared with the control group, the mRNA levels of Ngn1, ascl1a, HuC, and lslet1 in Sox19b MO-injection embryos increased significantly, but the mRNA level of her3 decreased significantly. b The expression levels of Ngn1, HuC, and lslet1 were detected by whole-mount in situ hybridization. Bar graphs show the percentage of normal embryos in each group. Data represent the mean of at least three independent experiments ± SD. *P < 0.05, **P < 0.01 versus control. c Quantitative RT-PCR analysis is shown for p21, p27, and p57. Knockdown of Sox19b caused a significant increase in the mRNA levels of cyclin-dependent kinase inhibitor. Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control. d Control and Sox19b MO-injected embryos were analysed by immunofluorescence staining of PHH3 (a marker of mitosis, red). Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control

Expression Data

Genes:	ascl1a cdkn1a cdkn1bb cdkn1ca elavl3 her3 isl1a neurog1
Antibody:	Ab36-h3
Fish:	WT WT + MO6-sox19b WT + MO7-sox19b
Knockdown Reagents:	MO6-sox19b MO7-sox19b
Anatomical Terms:	neural plate neural plate cell whole organism
Stage Range:	Bud to 1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO6-sox19b WT + MO7-sox19b
Knockdown Reagents:	MO6-sox19b MO7-sox19b
Observed In:	neural plate neural plate cell whole organism
Stage Range:	Bud to 1-4 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Stem Cell Res. Ther.