ZFIN ID: ZDB-FIG-180621-48
Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish. Frontiers in molecular neuroscience   11:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 5

Lpar2b regulates Yap1 activity. (A,A) Whole-mount ISH detects the mRNA of yap1 in embryos at 34 hpf. (A) High-magnification of the pLLP in (A). (B,C) Epifluorescence images of control- or yap1 MO-injected Et(gata2:EGFP)mp189b embryos at 48 hpf. (D) Quantification of the NM number at 48 hpf and number of pLLP cells at 32 hpf in control- or yap1 MO-injected embryos. (E–F) Confocal images of the pLLP stained with DAPI (blue) and p-Yap1 (green) at 32 hpf. (G,H) ISH shows a decreased prox1a expression in the pLLP of Lpar2b morphants. The pLLP is outlined by dots. **p < 0.01 compared to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf551Tg control Long-pec posterior lateral line neuromast IFL
zf551Tg + MO2-yap1 standard conditions Long-pec posterior lateral line neuromast IFL
prox1a AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
yap1 AB standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-yap1 IHC AB control Prim-15 Not Detected posterior lateral line primordium
IHC AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium prox1a expression decreased amount, abnormal
Prim-15 posterior lateral line primordium prox1a expression decreased distribution, abnormal
zf551Tg + MO2-yap1 standard conditions Prim-15 posterior lateral line primordium has fewer parts of type cell, abnormal
Long-pec whole organism has fewer parts of type posterior lateral line neuromast, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.