ZFIN ID: ZDB-FIG-180621-50
Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish. Frontiers in molecular neuroscience   11:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. S1

Validation of the efficacy of the MOs used in the study. (A-­‐B) Fluorescence images showing 8-­‐hpf embryos injected with the Lpar2bGFP construct (A) alone, or together with lpar2b MO (B). (C,D) Epifluorescence images of control MO-­‐ or lpar2b MO2-­‐injected Et(gata2:EGFP)mp189b embryos at 48 hpf. (E) The splicing-­‐blocking lpar2b MO2 disrupts normal slicing of the lpar2b pre-­‐mRNA as shown by RT-­‐PCR. (F) Quantification of the number of NMs at 48 hpf in (C,D). *, p < 0.05 compared to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf551Tg control Long-pec posterior lateral line neuromast IFL
zf551Tg + MO3-lpar2b standard conditions Long-pec posterior lateral line neuromast IFL
lpar2b AB control Long-pec whole organism RTPCR
zf551Tg + MO3-lpar2b standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf551Tg + MO3-lpar2b standard conditions Long-pec whole organism has fewer parts of type posterior lateral line neuromast, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.