ZFIN ID: ZDB-FIG-180621-45
Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish. Frontiers in molecular neuroscience   11:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

MO-mediated Lpar2b knock-down reduced the number of NMs and hair cells in the posterior lateral line. (A,B) Epifluorescence images of 48 hpf Et(gata2:EGFP)mp189b embryos injected with control or lpar2b MO. (C) Quantification of the number of trunk NMs (exclude terminal NMs) in one side of the pLL in (A,B). (D) Co-injection of MO-insensitive Lpar2b mRNA rescued the NMs phenotype caused by Lpar2b depletion. (E,F) Epifluorescence images of 72 hpf Tg(Brn3c:mGFP) embryos injected with control or lpar2b MO. (E,F) High-magnification images of hair cells in the L1 NMs in (E,F). (G) Quantification of the total number of hair cells in one side of the pLL (L1-L5) at 72 hpf. (H) Average number of hair cells per NM (L1-L5) at 72 hpf. (I,J) Snapshots from 6-h epifluorescence time-lapses movies of control or lpar2b MO-injected Tg(-8.0cldnb:lynEGFP) embryos at 30–36 hpf (Supplementary Movies 1, 2). (K) Quantification of the migration speed of the pLLP of (I,J). *p < 0.05; **p < 0.01; #p > 0.05 compared to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s356tTg control Protruding-mouth posterior lateral line neuromast hair cell IFL
s356tTg + MO2-lpar2b standard conditions Protruding-mouth posterior lateral line neuromast hair cell IFL
zf551Tg control Long-pec posterior lateral line neuromast IFL
zf551Tg + MO2-lpar2b standard conditions Long-pec posterior lateral line neuromast IFL
Long-pec posterior lateral line neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + MO2-lpar2b standard conditions Protruding-mouth posterior lateral line neuromast has fewer parts of type hair cell, abnormal
zf551Tg + MO2-lpar2b standard conditions Long-pec whole organism has fewer parts of type posterior lateral line neuromast, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.