| ZFIN ID: ZDB-FIG-180621-49 |
| Gene: | |
|---|---|
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Term: | |
| Stage: | Long-pec |
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Long-pec |
Fig. 6 A phosphorylation-resistant Yap1 mRNA (caYap1) rescued the pLLP and NM phenotype of Lpar2b morphants. (A–C″) Confocal images of the pLLP stained with DAPI (blue) and BrdU (red) at 32 hpf in Et(gata2:EGFP)mp189b embryos injected with control MO (A–A″), lpar2b MO (B–B″) or lpar2b MO and caYap1 mRNA together (C–C″). The pLLP is outlined by dots. (D–F) Epifluorescence image s of Et(gata2:EGFP)mp189b embryos injected with control MO (D), lpar2b MO (E), or lpar2b MO and caYap1 mRNA together (F) at 48 hpf. (G–I) Quantification of the total number of cells (G) and the number of BrdU+ cells (H) in the pLLP at 32 hpf, and the number of NMs at 48 hpf (I) in 3 groups of embryos as indicated. *p < 0.05; **p < 0.01. |
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| EGFP | zf551Tg | control | Long-pec | posterior lateral line neuromast | IFL | |
| zf551Tg + MO2-lpar2b | standard conditions | Long-pec | posterior lateral line neuromast | IFL |
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| zf551Tg + MO2-lpar2b | standard conditions | Long-pec | whole organism has fewer parts of type posterior lateral line neuromast, abnormal |
| Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci. |
|---|