FIGURE

Fig. S2

ID

ZDB-FIG-180621-51

Publication

Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish

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Fig. S2

Genetic mutation of lpar2b generated by the CRISPR-‐Cas9 system produces a similar NM phenotype in F0 embryos. (A) The gRNA was designed against the 5’ region of exon 3 of lpar2b genomic DNA. (B) Sequencing of the genomic DNA extracted from F0 embryos injected with the gRNA and Cas9 mRNA. (C) Fluorescence images of control Et(gata2:EGFP)^mp189b embryos injected with Cas9 mRNA alone, and embryos injected with lpar2b gRNA and Cas9 together at 48 hpf. (D) Quantification of the percentage of embryos with normal pLLP migration but fewer NMs (< 5 trunk NMs) at 48 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.