ZFIN ID: ZDB-FIG-180621-46
Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish. Frontiers in molecular neuroscience   11:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 3

Lpar2b regulates the patterning of the pLLP. (A) Confocal images showing the rosette structures in the pLLP of Tg(-8.0cldnb:lynEGFP) embryos at 32 hpf. (B) Immunostaining of ZO1 (red) in the pLLP of Tg(-8.0cldnb:lynEGFP) embryos at 32 hpf. Arrowheads mark the center of rosettes in (A,B). (C) Whole-mount ISH shows the expression of genes as indicated in the pLLP at 30–32 hpf in embryos injected with control or lpar2b MO. (D) Whole-mount ISH detects the expression of pea3 in the pLLP and midbrain-hindbrain boundary (MHB) regions in wild-type (WT) and Tg(hsp70:caFGFR1) embryos at 32 hpf. The Tg(hsp70:caFGFR1) embryos were heat shocked at 24 hpf for 1 h at 37°C. The pLLP is outlined by dots in (C,D). (E) Epifluorescence images showing Tg(-8.0cldnb:lynEGFP) /Tg(hsp70:caFGFR1) double transgenic embryos injected with lpar2b MO. Upper panel, without heat shock; lower panel, with heat shock. Arrowheads, a trail of cells left by pLLP. (F,G) Quantification of the area expressing pea3 (F) or lef1 (G) in the pLLP. **p < 0.01; #p > 0.05 compared to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ackr3b AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
cxcr4b AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
EGFP zf106Tg control Prim-15 posterior lateral line primordium IHC
zf106Tg + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium IHC
etv4 AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
fgf10a AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
fgfr1a AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
lef1 AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC zf106Tg control Prim-15 posterior lateral line primordium
IHC zf106Tg + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ackr3b expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium cxcr4b expression decreased amount, abnormal
Prim-15 posterior lateral line primordium etv4 expression decreased amount, abnormal
Prim-15 posterior lateral line primordium etv4 expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium fgf10a expression increased distribution, abnormal
Prim-15 posterior lateral line primordium fgfr1a expression decreased amount, abnormal
Prim-15 posterior lateral line primordium fgfr1a expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium lef1 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium decreased size, abnormal
zf106Tg + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in molecular neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Mol. Neurosci.