ZFIN ID: ZDB-FIG-180621-46
Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish. Frontiers in molecular neuroscience   11:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 3

Lpar2b regulates the patterning of the pLLP. (A) Confocal images showing the rosette structures in the pLLP of Tg(-8.0cldnb:lynEGFP) embryos at 32 hpf. (B) Immunostaining of ZO1 (red) in the pLLP of Tg(-8.0cldnb:lynEGFP) embryos at 32 hpf. Arrowheads mark the center of rosettes in (A,B). (C) Whole-mount ISH shows the expression of genes as indicated in the pLLP at 30–32 hpf in embryos injected with control or lpar2b MO. (D) Whole-mount ISH detects the expression of pea3 in the pLLP and midbrain-hindbrain boundary (MHB) regions in wild-type (WT) and Tg(hsp70:caFGFR1) embryos at 32 hpf. The Tg(hsp70:caFGFR1) embryos were heat shocked at 24 hpf for 1 h at 37°C. The pLLP is outlined by dots in (C,D). (E) Epifluorescence images showing Tg(-8.0cldnb:lynEGFP) /Tg(hsp70:caFGFR1) double transgenic embryos injected with lpar2b MO. Upper panel, without heat shock; lower panel, with heat shock. Arrowheads, a trail of cells left by pLLP. (F,G) Quantification of the area expressing pea3 (F) or lef1 (G) in the pLLP. **p < 0.01; #p > 0.05 compared to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ackr3b AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
cxcr4b AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
EGFP zf106Tg control Prim-15 posterior lateral line primordium IHC
zf106Tg + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium IHC
etv4 AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
fgf10a AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
fgfr1a AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
lef1 AB control Prim-15 posterior lateral line primordium ISH
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC zf106Tg control Prim-15 posterior lateral line primordium
IHC zf106Tg + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ackr3b expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium cxcr4b expression decreased amount, abnormal
Prim-15 posterior lateral line primordium etv4 expression decreased amount, abnormal
Prim-15 posterior lateral line primordium etv4 expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium fgf10a expression increased distribution, abnormal
Prim-15 posterior lateral line primordium fgfr1a expression decreased amount, abnormal
Prim-15 posterior lateral line primordium fgfr1a expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium lef1 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium decreased size, abnormal
zf106Tg + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium decreased size, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.