|ZFIN ID: ZDB-FIG-180621-47|
Lpar2b regulates the tissue size of the pLLP. (A) Confocal images showing the pLLP of control- or lpar2b MO-injected Et(gata2:EGFP)mp189b embryos at 32 hpf. The embryos were stained with DAPI to show cell nuclei (blue). GFP comes from the gata2:EGFP transgene. The pLLP is outlined by dots. (B) Quantification of the total number of cells in the pLLP. (C) Confocal images of the pLLP at 32 hpf after DAPI (blue) and BrdU (red) staining. (D) Quantification of the number of BrdU+ cells in the pLLP. (E) The BrdU index is calculated as (number of BrdU+ cells in pLLP)/(total number of pLLP cells). Con-MO, Control MO; **p < 0.01; #p > 0.05 compared to control.
|EGFP||zf551Tg||control||Prim-15||posterior lateral line primordium||IFL|
|zf551Tg + MO2-lpar2b||standard conditions||Prim-15||posterior lateral line primordium||IFL|
|zf551Tg + MO2-lpar2b||standard conditions||Prim-15||posterior lateral line primordium EGFP expression decreased distribution, abnormal|
|Prim-15||posterior lateral line primordium has fewer parts of type cell, abnormal|
|Acknowledgments:||This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.|