ZFIN ID: ZDB-FIG-180621-53
Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish. Frontiers in molecular neuroscience   11:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. S4

Validation of the p-­‐Yap1 antibody used in the study. (A) Alignment of the N-­‐terminal amino acid sequences of human (hsYAP1) and zebrafish Yap1 (drYap1) proteins. High concensus sequences (>90%) are shown in red, and low concensus sequences (>50%) are depicted in blue. Arrow, the Ser127 phosphorylation site recognized by the antibody. (B)Confocal images of the pronephric duct region stained with p-­‐Yap1 and DAPI in 32 hpf embryo injected with control-­‐ of yap1 MO. Images in the left 2 panels are generated from maximum projection of 6 confocal z-­‐stacks. A single z-­‐stack of the boxed areas is depicted in the right 3 panels. Note the p-­‐Yap1 signal is in the cytoplasm and does not overlap with the nucleus. (C) Confocal images of the pLLP cells stained with p-­‐Yap1 and DAPI in embryos injected with indicated MOs. The p-­‐Yap1 signal is in the cytosol of pLLP cells.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-yap1 IHC AB control Prim-15 posterior lateral line primordium
IHC Prim-15 pronephric duct
IHC AB + MO2-lpar2b standard conditions Prim-15 Not Detected posterior lateral line primordium
IHC Prim-15 Not Detected pronephric duct
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-lpar2b standard conditions Prim-15 posterior lateral line primordium ab1-yap1 labeling absent, abnormal
Prim-15 pronephric duct ab1-yap1 labeling absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.