|ZFIN ID: ZDB-FIG-180621-53|
Validation of the p-‐Yap1 antibody used in the study. (A) Alignment of the N-‐terminal amino acid sequences of human (hsYAP1) and zebrafish Yap1 (drYap1) proteins. High concensus sequences (>90%) are shown in red, and low concensus sequences (>50%) are depicted in blue. Arrow, the Ser127 phosphorylation site recognized by the antibody. (B)Confocal images of the pronephric duct region stained with p-‐Yap1 and DAPI in 32 hpf embryo injected with control-‐ of yap1 MO. Images in the left 2 panels are generated from maximum projection of 6 confocal z-‐stacks. A single z-‐stack of the boxed areas is depicted in the right 3 panels. Note the p-‐Yap1 signal is in the cytoplasm and does not overlap with the nucleus. (C) Confocal images of the pLLP cells stained with p-‐Yap1 and DAPI in embryos injected with indicated MOs. The p-‐Yap1 signal is in the cytosol of pLLP cells.
|Ab1-yap1||IHC||AB||control||Prim-15||posterior lateral line primordium|
|IHC||AB + MO2-lpar2b||standard conditions||Prim-15||Not Detected||posterior lateral line primordium|
|IHC||Prim-15||Not Detected||pronephric duct|
|AB + MO2-lpar2b||standard conditions||Prim-15||posterior lateral line primordium ab1-yap1 labeling absent, abnormal|
|Prim-15||pronephric duct ab1-yap1 labeling absent, abnormal|
|Acknowledgments:||This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.|