ZFIN ID: ZDB-FIG-171018-14
Leerberg et al., 2017 - Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish. PLoS Genetics   13:e1006993 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 5

Fgf24 regulates gene expression of somatic gonad cells.

(A-J) Single plane confocal micrographs of whole-mount larval gonads after fluorescent in situ hybridization detecting SGC markers (red). Nuclei labeled with DAPI (blue), germ cells labeled with Vasa (green). gata4 (A), nr5a1a (C), wt1a (E), cyp19a1a (G), and amh (I) are expressed in somatic gonad cells (SGCs) of wild-type larval gonads, whereas only wt1a can be detected in SGCs of fgf24 mutants (F). (K-M) Double fluorescent in situ hybridization and Vasa antibody staining (blue) of 12 dpf wild-type gonads (outlined by white dotted line). etv4, cyp19a1a, and amh are expressed in non-overlapping SGC domains (n = 12, 12, and 3 for K, L, and M, respectively). In E, arrows denote inner SGCs; arrowheads denote outer SGCs. A-M are sagittal optical sections with anterior to the left. Scale bars = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
amh AB standard conditions Days 7-13 gonad primordium somatic cell ISH
fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell ISH
cyp19a1a AB standard conditions Days 7-13 gonad primordium somatic cell ISH
fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell ISH
ddx4 Ab1-ddx4 AB standard conditions Days 7-13 germ line cell IHC
Ab1-ddx4 fgf24uc47/uc47(AB) standard conditions Days 7-13 germ line cell IHC
etv4 AB standard conditions Days 7-13 gonad primordium somatic cell ISH
gata4 AB standard conditions Days 7-13 gonad primordium somatic cell ISH
fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell ISH
nr5a1a AB standard conditions Days 7-13 gonad primordium somatic cell ISH
fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell ISH
wt1a AB standard conditions Days 7-13 gonad primordium somatic cell ISH
fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell amh expression decreased amount, abnormal
Days 7-13 gonad primordium somatic cell cyp19a1a expression decreased amount, abnormal
Days 7-13 gonad primordium somatic cell gata4 expression decreased amount, abnormal
Days 7-13 gonad primordium somatic cell nr5a1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.