ZFIN ID: ZDB-FIG-171018-16
Leerberg et al., 2017 - Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish. PLoS Genetics   13:e1006993 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 7

fgf24 mutant gonads lack wild-type organization.

(A-B'') Transmission electron micrographs of 10 dpf gonads, transverse sections. In both wild-type (WT) and fgf24 mutant gonads, germ cells (pseudocolored green) are enclosed by SGCs (A, B). However, the somatic gonad component of the wild-type gonad is thicker than in the fgf24 mutant gonad, and is split into two layers by an electron-lucent space (arrowheads in A and A', which is a higher magnification of the box in A). The somatic gonad component is single layered, as shown at higher magnification in B' (corresponds to the box in B). Somatic gonad cells extend processes and make cell-cell contacts (arrows) in both wild-type and fgf24 mutant gonads (A'', B'', respectively). (C-D') Antibody staining against Laminin (orange) and Vasa (green) of 10 dpf gonads; individual germ cells are outlined with dotted white lines. Laminin is deposited between nuclei of SGCs in wild-type (C, C') but not fgf24 mutant (D, D') gonads. C-D' are sagittal optical sections with anterior to the left. Nuclei are labeled with DAPI (blue). Scale bars = 2 μm in A, B; 1 μm in A', A'', B', B''; 5 μm in C-D'.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx4 Ab1-ddx4 AB standard conditions Days 7-13 gonad primordium germ line cell IHC
Ab1-ddx4 fgf24uc47/uc47 standard conditions Days 7-13 gonad primordium germ line cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-lama1 IHC AB standard conditions Days 7-13 gonad primordium
IHC fgf24uc47/uc47 standard conditions Days 7-13 Not Detected gonad primordium
Phenotype Details
Fish Conditions Stage Phenotype
fgf24uc47/uc47 standard conditions Days 7-13 gonad primordium ab1-lama1 labeling absent, abnormal
Days 7-13 gonad primordium somatic cell morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.