Gene
ddx4
- ID
- ZDB-GENE-990415-272
- Name
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
- Symbol
- ddx4 Nomenclature History
- Previous Names
-
- vas
- vasa
- vlg
- wu:fi24g05
- zgc:109812
- zgc:158535
- Type
- protein_coding_gene
- Location
- Chr: 10 Mapping Details/Browsers
- Description
- Predicted to enable RNA helicase activity and mRNA binding activity. Acts upstream of or within germ cell development; gonad development; and regulation of meiotic cell cycle. Located in P granule. Is expressed in several structures, including blastodisc; germ line cell; reproductive system; shield; and unfertilized egg. Orthologous to human DDX4 (DEAD-box helicase 4).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 180 figures from 101 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- 6 figures from Hartung et al., 2014
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| sa6158 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa9472 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa15900 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa18973 | Allele with one point mutation | Unknown | Premature Stop | ENU |
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Human Disease
Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Conserved_site | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| Domain | IPR001650 | Helicase, C-terminal domain-like |
| Domain | IPR011545 | DEAD/DEAH box helicase domain |
| Domain | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| Domain | IPR014014 | RNA helicase, DEAD-box type, Q motif |
| Homologous_superfamily | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
Domain Details Per Protein
| Protein | Additional Resources | Length | ATP-dependent RNA helicase DEAD-box, conserved site | DEAD/DEAH box helicase domain | Helicase, C-terminal domain-like | Helicase superfamily 1/2, ATP-binding domain | P-loop containing nucleoside triphosphate hydrolase | RNA helicase, DEAD-box type, Q motif |
|---|---|---|---|---|---|---|---|---|
| UniProtKB:O42107 | InterPro | 716 | ||||||
| UniProtKB:A0A8M9PTC4 | InterPro | 700 | ||||||
| UniProtKB:A0A8M6Z2W1 | InterPro | 714 | ||||||
| UniProtKB:A0A8M6Z8Q8 | InterPro | 704 | ||||||
| UniProtKB:A0A8M9Q5S2 | InterPro | 699 | ||||||
| UniProtKB:Q8QGG8 | InterPro | 715 | ||||||
| UniProtKB:A0A8M9QBU4 | InterPro | 688 | ||||||
| UniProtKB:A0A8M9QKJ0 | InterPro | 687 | ||||||
| UniProtKB:A0AB32TXW6 | InterPro | 687 | ||||||
| UniProtKB:A0AB32U0J5 | InterPro | 699 |
Interactions and Pathways
No data available
Plasmids
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(ddx4:ddx4-EGFP) |
|
|
| 2 | (58) |
| Tg(ddx4:Dendra2-NTR-UTR) |
|
| 1 | Cao et al., 2019 | |
| Tg(ddx4:dnraraa-IRES-mCherry,myl7:EGFP) |
|
| 1 | Crespo et al., 2019 | |
| Tg(ddx4:DsRed2-ddx4) |
|
|
| 1 | (5) |
| Tg(ddx4:EGFP) |
|
| 1 | Franěk et al., 2019 | |
| Tg(ddx4:GFP) |
|
| 1 | (2) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-194F17 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-117I20 | ZFIN Curated Data | |
| Encodes | EST | fi24g05 | ||
| Encodes | cDNA | MGC:109812 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:158535 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_131057 (1) | 2865 nt | ||
| Genomic | GenBank:BX571726 (2) | 106819 nt | ||
| Polypeptide | UniProtKB:O42107 (1) | 716 aa |
- Carver, J.J., Amato, C.M., Yao, H., Zhu, Y. (2024) Adamts9 is required for the development of primary ovarian follicles and maintenance of female sex in zebrafish. Biology of reproduction. 111(5):1107-1128
- Casey, M.J., Chan, P.P., Li, Q., Zu, J.F., Jette, C.A., Kohler, M., Myers, B.R., Stewart, R.A. (2024) A simple and scalable zebrafish model of Sonic hedgehog medulloblastoma. Cell Reports. 43:114559114559
- Chen, J., Zhao, W., Cao, L., Martins, R.S.T., Canário, A.V.M. (2024) Somatostatin signalling coordinates energy metabolism allocation to reproduction in zebrafish. BMC Biology. 22:163163
- Chu, W.K., Huang, S.C., Chang, C.F., Wu, J.L., Gong, H.Y. (2023) Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of the dnd1 gene by CRISPR/Cas9 genome editing. Frontiers in genetics. 14:10292001029200
- Islam, K.N., Ajao, A., Venkataramani, K., Rivera, J., Pathania, S., Henke, K., Siegfried, K.R. (2023) The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish. PLoS Genetics. 19:e1010589e1010589
- Li, W., Liu, W., Mo, C., Yi, M., Gui, J. (2023) Two Novel lncRNAs Regulate Primordial Germ Cell Development in Zebrafish. Cells. 12(4):
- Ramachandran, D., Sharma, K., Saxena, V., Nipu, N., Rajapaksha, D.C., Mennigen, J.A. (2023) Knock-out of vasotocin reduces reproductive success in female zebrafish, Danio rerio. Frontiers in endocrinology. 14:11512991151299
- Wang, X., Zhu, J., Wang, H., Deng, W., Jiao, S., Wang, Y., He, M., Zhang, F., Liu, T., Hao, Y., Ye, D., Sun, Y. (2023) Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors. Nature communications. 14:79187918
- Ye, D., Liu, T., Li, Y., Wang, Y., Hu, W., Zhu, Z., Sun, Y. (2023) Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set. Frontiers in endocrinology. 14:10443181044318
- Annus, T., Müller, D., Jezsó, B., Ullaga, G., Németh, B., Harami, G.M., Orbán, L., Kovács, M., Varga, M. (2022) Bloom syndrome helicase contributes to germ line development and longevity in zebrafish. Cell Death & Disease. 13:363
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