ZFIN ID: ZDB-FIG-171018-17
Leerberg et al., 2017 - Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish. PLoS Genetics   13:e1006993 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 8

Somatic cells of wild-type and fgf24 mutant gonads express epithelial-like cell adhesion molecules.

(A-F') Single plane confocal micrographs of whole-mount larval gonads immunostained for cell adhesion molecules (red). (A-B') β-catenin localizes to the membranes of both SGCs and germ cells in wild-type (WT) gonads (A, A'). While β-catenin still localizes to the membranes of both cell types in fgf24 mutants, it appears reduced (B, B'). (C-D') Cdh2 localizes strongly to the outer layer of SGCs in wild-type and fgf24 mutant gonads, visible on the surface of the gonad (C, D) and in the external-most layer of SGCs of the interior view (C', D'). (E-F') Similar to Cdh2, Tjp1 localizes to the outer layer of SGCs in wild-type (E, E') and fgf24 mutant (F, F') gonads. A-F' are sagittal optical sections with anterior to the left. Germ cells are labeled with Vasa (green), nuclei are labeled with DAPI (blue). Scale bars = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx4 Ab1-ddx4 AB standard conditions Days 7-13 gonad primordium germ line cell IHC
Ab1-ddx4 fgf24uc47/uc47 (AB) standard conditions Days 7-13 gonad primordium germ line cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC AB standard conditions Days 7-13 gonad primordium germ line cell
IHC Days 7-13 gonad primordium somatic cell
IHC fgf24uc47/uc47 (AB) standard conditions Days 7-13 gonad primordium germ line cell
IHC Days 7-13 gonad primordium somatic cell
Ab1-tjp1 IHC AB standard conditions Days 7-13 gonad primordium somatic cell
IHC fgf24uc47/uc47 (AB) standard conditions Days 7-13 gonad primordium somatic cell
Ab6-cdh2 IHC AB standard conditions Days 7-13 gonad primordium somatic cell
IHC fgf24uc47/uc47 (AB) standard conditions Days 7-13 gonad primordium somatic cell
Phenotype Details
Fish Conditions Stage Phenotype
fgf24uc47/uc47 (AB) standard conditions Days 7-13 gonad primordium germ line cell ab1-ctnnb labeling decreased amount, abnormal
Days 7-13 gonad primordium somatic cell ab1-ctnnb labeling decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.