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ZFIN ID: ZDB-FIG-171018-17
Leerberg et al., 2017 - Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish. PLoS Genetics   13:e1006993 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 8

Somatic cells of wild-type and fgf24 mutant gonads express epithelial-like cell adhesion molecules.

(A-F') Single plane confocal micrographs of whole-mount larval gonads immunostained for cell adhesion molecules (red). (A-B') β-catenin localizes to the membranes of both SGCs and germ cells in wild-type (WT) gonads (A, A'). While β-catenin still localizes to the membranes of both cell types in fgf24 mutants, it appears reduced (B, B'). (C-D') Cdh2 localizes strongly to the outer layer of SGCs in wild-type and fgf24 mutant gonads, visible on the surface of the gonad (C, D) and in the external-most layer of SGCs of the interior view (C', D'). (E-F') Similar to Cdh2, Tjp1 localizes to the outer layer of SGCs in wild-type (E, E') and fgf24 mutant (F, F') gonads. A-F' are sagittal optical sections with anterior to the left. Germ cells are labeled with Vasa (green), nuclei are labeled with DAPI (blue). Scale bars = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx4 Ab1-ddx4 AB standard conditions Days 7-13 gonad primordium germ line cell IHC
Ab1-ddx4 fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium germ line cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC AB standard conditions Days 7-13 gonad primordium germ line cell
IHC Days 7-13 gonad primordium somatic cell
IHC fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium germ line cell
IHC Days 7-13 gonad primordium somatic cell
Ab1-tjp1 IHC AB standard conditions Days 7-13 gonad primordium somatic cell
IHC fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell
Ab6-cdh2 IHC AB standard conditions Days 7-13 gonad primordium somatic cell
IHC fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium somatic cell
Phenotype Details
Fish Conditions Stage Phenotype
fgf24uc47/uc47(AB) standard conditions Days 7-13 gonad primordium germ line cell ab1-ctnnb labeling decreased amount, abnormal
Days 7-13 gonad primordium somatic cell ab1-ctnnb labeling decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.