ZFIN ID: ZDB-FIG-171018-13
Leerberg et al., 2017 - Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish. PLoS Genetics   13:e1006993 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 4

A second, inner layer of somatic gonad cells responds to Fgf24 signaling.

(A-D, E) Single plane view of whole mount gonads after fluorescent in situ hybridization. (A-B) At 8 dpf, ~85% of wild-type (WT) fish express fgf24 (red) in SGCs, whereas ~56% express etv4 (green) (A). (C) etv4 is undetected in fgf24 mutant gonads (n = 14). (D) By 10 dpf, SGCs located externally express fgf24, whereas an inner layer of SGCs expresses etv4. (D') An orthogonal view of a Z stack reconstruction of the gonad in D (white line). (E) In 10 dpf fgf24 mutant gonads, etv4 is expressed at low levels or not at all. Germ cells labeled with Vasa (blue). Gonads are outlined with white dotted lines in A-C. (F-G) Antibody staining of 10 dpf gonads detecting phosphorylated Erk (pErk, red). Nuclei labeled with DAPI (blue), germ cells labeled with Vasa (green). SGCs of wild-type gonads (F), but not fgf24 mutant gonads (G), show Erk phosphorylation. (F', G') are pErk channel only of (F) and (G), respectively. A-D, E-G' are sagittal optical sections with anterior to the left. Scale bars = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx4 Ab1-ddx4 AB standard conditions Days 7-13 germ line cell IHC
Ab1-ddx4 fgf24uc47/uc47 standard conditions Days 7-13 germ line cell IHC
etv4 AB standard conditions Days 7-13 gonad primordium somatic cell ISH
fgf24uc47/uc47 standard conditions Days 7-13 gonad primordium somatic cell ISH
fgf24 AB standard conditions Days 7-13 gonad primordium somatic cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-mapk IHC AB standard conditions Days 7-13 gonad primordium somatic cell
IHC fgf24uc47/uc47 standard conditions Days 7-13 Not Detected gonad primordium somatic cell
Phenotype Details
Fish Conditions Stage Phenotype
fgf24uc47/uc47 standard conditions Days 7-13 gonad primordium somatic cell ab2-mapk labeling absent, abnormal
Days 7-13 gonad primordium somatic cell etv4 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.