ZFIN ID: ZDB-FIG-171018-11
Leerberg et al., 2017 - Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish. PLoS Genetics   13:e1006993 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Days 7-13 to Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Adult

Fig. 2

Larval and juvenile fgf24 mutants have severely underdeveloped gonads and consequently develop as male.

(A-C') fgf24 mutants are phenotypically male. A wild-type (WT) female has a distended belly (A) and protruding cloaca (arrow, A') compared to the slender belly and obscured cloaca of the wild-type (B, B') and mutant (C, C') males. Note the absence of pectoral fin in the fgf24 mutant (C) compared to wild-type animals (arrowheads, A, B). (D) Box and whisker plot representing the number of germ cells per wild-type and fgf24 mutant fish during larval development. Each dot represents the number of germ cells in one animal (Unpaired two-tailed t-test; n = 15 for both 8 dpf wild-type and mutant, n = 13 for 10 dpf wild-type, n = 12 for 10 dpf mutant, n = 10 for 12 dpf wild-type, n = 11 for 12 dpf mutant, n = 16 for 14 dpf wild-type, n = 12 for 14 dpf mutant; * = P < .05; n.s. = no significance). (E-H') Representative confocal images of 12 dpf (E-F) and 40 dpf (G-H') wild-type (E, G-G') and fgf24 mutant (F, H-H') gonads. At 12 dpf, gonads of fgf24 mutants (F) have fewer Vasa positive germ cells (green) compared to wild-type (E). By 40 dpf, wild-type testes have many Tg(ziwi:EGFP) positive germ cells (green), both premeiotic and spermatogenic, that are enclosed by Tg(gsdf:mCherry) positive Sertoli cells (red) (G-G'). In contrast, gonads of 40 dpf fgf24 mutants are unorganized, lack Tg(gsdf:mCherry) expressing Sertoli cells, and contain few germ cells, all of which are premeiotic (H-H'). G' and H' are magnified views G and H, respectively. The boxed nuclei in G' and H' are magnified in the respective insets, with an arrow indicating the large nucleoli (DAPI only, in grey). E-H' are sagittal optical sections with anterior to the left. Nuclei labeled with DAPI (blue). Scale bars = 20 μm for E, F, G, H; 100 μm for G', H'.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ddx4 Ab1-ddx4 AB standard conditions Days 7-13 gonad primordium germ line cell IHC
Ab1-ddx4 Days 14-20 immature gonad germ line cell IHC
Ab1-ddx4 fgf24uc47/uc47 (AB) standard conditions Days 7-13 gonad primordium germ line cell IHC
Ab1-ddx4 Days 14-20 immature gonad germ line cell IHC
EGFP fgf24uc47/uc47; uc02Tg; uc46Tg standard conditions Days 30-44 gonad germ line cell IFL
uc02Tg; uc46Tg standard conditions Days 30-44 gonad germ line cell IFL
mCherry fgf24uc47/uc47; uc02Tg; uc46Tg standard conditions Days 30-44 Not Detected Sertoli cell IFL
uc02Tg; uc46Tg standard conditions Days 30-44 Sertoli cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf24t22030/t22030 standard conditions Days 7-13 germ line cell decreased amount, abnormal
Days 7-13 gonad primordium decreased size, abnormal
Days 14-20 germ line cell decreased amount, abnormal
Adult female organism absent, abnormal
Adult whole organism lacks all parts of type pectoral fin, abnormal
fgf24uc47/uc47 (AB) standard conditions Days 7-13 gonad primordium germ line cell ddx4 expression decreased amount, abnormal
Days 14-20 immature gonad germ line cell ddx4 expression decreased amount, abnormal
fgf24uc47/uc47; uc02Tg; uc46Tg standard conditions Days 30-44 germ line cell decreased amount, abnormal
Days 30-44 gonad disorganized, abnormal
Days 30-44 gonad germ line cell EGFP expression decreased amount, abnormal
Days 30-44 Sertoli cell absent, abnormal
Days 30-44 Sertoli cell mCherry expression absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.