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ZFIN ID: ZDB-FIG-170519-24
Lei et al., 2017 - Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors. Nucleic acids research   45(6):3422-3436 Full text @ Nucleic Acids Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Fig. 7

The p53 signaling pathway is activated in fn10a mutants. (A) Gene expression in RPKM (Reads Per Kilobase of transcript per Million mapped reads) in mutants and WT-siblings. Yellow asterisks, p53 pathway genes; red circles, genes of the p53 pathway differentially expressed in mutants and their siblings; green circles, intron-retained genes in the p53 pathway; and blue circles, other RefSeq genes. (B) Genes of the p53 pathway were up-regulated and enriched with intron retention but few had both intron retention and increased mRNA levels in fn10a mutants. (C) Real-time PCR confirmed the transcriptional up-regulation of p53 pathway genes (n = 5). (D) Knockdown of p53 expression in fn10a mutants rescued neuronal apoptosis in the CNS and spinal cord. Note that ∼50% of p53MO-injected fn10a mutants had no TUNEL signals and ∼42% had fewer TUNEL signals. (E) Over-expression of upf1 mRNA partially reduced p53 expression in eftud2 morphants while it had no effect on p53 expression in wild-type embryos (n = 5).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
baxa eftud2fn10a/fn10a(TL) standard conditions Prim-25 whole organism RTPCR
bbc3 eftud2fn10a/fn10a(TL) standard conditions Prim-25 whole organism RTPCR
casp8 eftud2fn10a/fn10a(TL) standard conditions Prim-25 whole organism RTPCR
diabloa eftud2fn10a/fn10a(TL) standard conditions Prim-25 whole organism RTPCR
gadd45aa eftud2fn10a/fn10a(TL) standard conditions Prim-25 whole organism RTPCR
mdm2 eftud2fn10a/fn10a(TL) standard conditions Prim-25 whole organism RTPCR
tp53 TL + MO1-eftud2 standard conditions Prim-25 whole organism RTPCR
eftud2fn10a/fn10a(TL) standard conditions Prim-25 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO1-eftud2 standard conditions Prim-25 whole organism tp53 expression increased amount, abnormal
eftud2fn10a/fn10a(TL) standard conditions Prim-25 brain apoptotic process increased occurrence, abnormal
Prim-25 intrinsic apoptotic signaling pathway by p53 class mediator increased occurrence, abnormal
Prim-25 spinal cord apoptotic process increased occurrence, abnormal
Prim-25 whole organism diabloa expression increased amount, abnormal
Prim-25 whole organism gadd45aa expression increased amount, abnormal
Prim-25 whole organism tp53 expression increased amount, abnormal
Prim-25 whole organism mdm2 expression increased amount, abnormal
Prim-25 whole organism bbc3 expression increased amount, abnormal
Prim-25 whole organism casp8 expression increased amount, abnormal
Prim-25 whole organism baxa expression increased amount, abnormal
eftud2fn10a/fn10a + MO4-tp53(TL) standard conditions Prim-25 brain apoptotic process occurrence, ameliorated
Prim-25 spinal cord apoptotic process occurrence, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Nucleic acids research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nucleic Acids Res.