ZFIN Image: Lei et al., 2017, Fig. 7

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Figure Caption

Fig. 7

The p53 signaling pathway is activated in fn10a mutants. (A) Gene expression in RPKM (Reads Per Kilobase of transcript per Million mapped reads) in mutants and WT-siblings. Yellow asterisks, p53 pathway genes; red circles, genes of the p53 pathway differentially expressed in mutants and their siblings; green circles, intron-retained genes in the p53 pathway; and blue circles, other RefSeq genes. (B) Genes of the p53 pathway were up-regulated and enriched with intron retention but few had both intron retention and increased mRNA levels in fn10a mutants. (C) Real-time PCR confirmed the transcriptional up-regulation of p53 pathway genes (n = 5). (D) Knockdown of p53 expression in fn10a mutants rescued neuronal apoptosis in the CNS and spinal cord. Note that ∼50% of p53MO-injected fn10a mutants had no TUNEL signals and ∼42% had fewer TUNEL signals. (E) Over-expression of upf1 mRNA partially reduced p53 expression in eftud2 morphants while it had no effect on p53 expression in wild-type embryos (n = 5).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.