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ZIRC
ZFIN ID: ZDB-FIG-131010-7
D'Aniello et al., 2013 - Depletion of Retinoic Acid Receptors Initiates a Novel Positive Feedback Mechanism that Promotes Teratogenic Increases in Retinoic Acid. PLoS Genetics   9(8):e1003689 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 3

RARαb1 deficient embryos have increased expression of RA signaling responsive genes.

(A) qPCR for RA signaling responsive gene expression at the 8 s stage. (B, C) ISH for hoxb5b expression at the 8 s stage. Bracket indicates length of expression in the LPM. Views are dorsal with anterior up. (D, E) ISH for cyp26a1 expression at the 8 s stage. Arrows in E indicate increased expression in the tailbud and spinal cord. Views are lateral with anterior up and dorsal right. (F–H) Fronto-lateral views of Tg(-5.1myl7:GFP)f2 embryos at 48 hpf of control sibling, RARαb1 deficient embryos, and RARαb1+Hoxb5b deficient embryos. (I–K) Hearts from control sibling, RARαb1 deficient embryos and RARαb1+Hoxb5b deficient Tg(-5.1myl7:DsRed-NLS)f2 embryos. Images are frontal views. Red indicates ventricle. Green indicates atrium. (L) Percentage of control+rarαb1 MOs (n = 60), hoxb5b+rarαb1 MOs (n = 68) showing enlarged and normal hearts. (M) qPCR for CM differentiation gene expression at 48 hpf. (N) Mean CM number at 48 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyp26a1 WT control 5-9 somites neural plate ISH
5-9 somites tail bud ISH
WT + MO2-rarab + MO4-tp53 standard conditions 5-9 somites neural plate ISH
5-9 somites tail bud ISH
DsRed2 f2Tg control Long-pec cardiac muscle cell nucleus IHC
f2Tg + MO1-hoxb5b + MO1-rarab + MO4-tp53 standard conditions Long-pec cardiac muscle cell nucleus IHC
f2Tg + MO2-rarab + MO4-tp53 standard conditions Long-pec cardiac muscle cell nucleus IHC
EGFP twu26Tg control Long-pec cardiac muscle cell IHC
twu26Tg + MO1-hoxb5b + MO1-rarab + MO4-tp53 standard conditions Long-pec cardiac muscle cell IHC
twu26Tg + MO2-rarab + MO4-tp53 standard conditions Long-pec cardiac muscle cell IHC
hoxb5b WT control 5-9 somites lateral plate mesoderm ISH
WT + MO2-rarab + MO4-tp53 standard conditions 5-9 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu26Tg + MO2-rarab + MO4-tp53 standard conditions Long-pec heart has extra parts of type cardiac muscle cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.