FIGURE

Fig. 6

ID

ZDB-FIG-131010-10

Publication

D'Aniello et al., 2013 - Depletion of Retinoic Acid Receptors Initiates a Novel Positive Feedback Mechanism that Promotes Teratogenic Increases in Retinoic Acid

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Reduction of RA in RARαb1 deficient embryos can rescue developmental defects.

(A–H) Control sibling, RARαb1+Cyp26a1 deficient, control sibling treated with DEAB, and RARαb1+Cyp26a1 treated with DEAB embryos. In B and F, arrows indicates loss of the MHB and eng2a expression. Images are lateral views with dorsal right and anterior up. (I) Percentage of control sibling (n = 16), RARαb1+Cyp26a1 deficient embryos (n = 16), control sibling embryos treated with DEAB (n = 13), and RARαb1+Cyp26a1 deficient embryos treated with DEAB (n = 14) that had a MHB based on morphology. (J) Percentage of control sibling (n = 17), RARαb1+Cyp26a1 deficient embryos (n = 14), control sibling embryos treated with DEAB (n = 15), and RARαb1+Cyp26a1 deficient embryos treated with DEAB (n = 12) that had eng2a expression at the MHB. (K–N) Hearts from Tg(-5.1myl7:DsRed-NLS)^f2 control sibling, RARαb1 deficient, DEAB treated, and DEAB+RARαb1deficient embryos. Images are frontal views. Red indicates ventricle. Green indicates atrium. (O) Mean CM number at 48 hpf. (P,Q) Hearts from Tg(-5.1myl7:DsRed-NLS)^f2 control sibling embryos and Tg(-5.1myl7:DsRed-NLS)^f2 embryos treated with a low concentration of RA. Images are frontal views. Red indicates ventricle. Green indicates atrium. (R) Mean CM number at 48 hpf.

Expression Data

Genes:	DsRed2 en2a
Fish:	WT f2Tg f2Tg + MO2-rarab + MO4-tp53 WT + MO2-rarab + MO4-cyp26a1 + MO4-tp53 + MO5-cyp26a1
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO2-rarab MO4-cyp26a1 MO4-tp53 MO5-cyp26a1
Anatomical Terms:	cardiac muscle cell nucleus midbrain hindbrain boundary
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f2Tg f2Tg + MO2-rarab + MO4-tp53 WT + MO2-rarab + MO4-cyp26a1 + MO4-tp53 + MO5-cyp26a1
Condition:	chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO2-rarab MO4-cyp26a1 MO4-tp53 MO5-cyp26a1
Observed In:	atrium cardiac muscle cell cardiac ventricle heart midbrain hindbrain boundary
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.