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ZFIN ID: ZDB-FIG-130802-42
Amali et al., 2013 - Zebrafish hoxd4a Acts Upstream of meis1.1 to Direct Vasculogenesis, Angiogenesis and Hematopoiesis. PLoS One   8(3):e58857 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5

Fig. 10

cdx4, meis1.1 and Hox gene expression in hoxd4a morphants, and a genetic pathway for specification of hemangioblasts and unipotential stem cells.

(A) qRT-PCR showing decreased expression of meis1.1 and many, but not all, hox genes in hoxd4a morphants at 26–28 hpf. By contrast, cdx4 levels are unchanged. Samples were normalized to ²-actin. Error bars indicate standard error. All pairs marked with an asterisk meet statistical significance (pd0.02). (B) Based on the results presented here and those in the literature, we propose a pathway in which hoxd4a and meis1.1 occupy sequential steps downstream of cloche and cdx1/4 in a genetic programme leading to the specification of hemangioblasts and unipotential angiogenic and hematopoietic stem cells. The effects of hoxd4a knockdown may be magnified through positive cross-regulatory interactions with meis1.1. The observed effects on the expression of multiple Hox genes could be due to the direct action of hoxd4a and meis1.1. Non-exclusively, cdx1 and cdx4 may act in conjunction with hoxd4a and meis1.1 in a feed-forward type of mechanism to regulate one or more of these same Hox genes with widespread consequences for vasculogenesis, angiogenesis and hematopoiesis at all levels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdx4 AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxa9a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxa10b AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxb1a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxb3a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxb4a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxb5a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxb6b AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxb7a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxb8a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxc4a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxc5a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxc6a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxc8a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxc9a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxd3a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
hoxd10a AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
meis1b AB control Prim-5 whole organism RTPCR
AB + MO1-hoxd4a standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One