ZFIN ID: ZDB-FIG-130210-7
Yin et al., 2012 - Inhibition of the pim1 oncogene results in diminished visual function. PLoS One   7(12):e52177 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Long-pec to Days 7-13

Fig. 3

Up-regulation of gene and protein expression in the Jak-Stat signaling pathway from 3 to 5 dpf during eye development.

(A) Red blocks are up-regulated genes between 5 versus 3 dpf. Green blocks are down-regulated genes between 5 versus 3 dpf. Grey blocks are genes not changed between 5 versus 3 dpf. White blocks are genes not targeted by the microarray or not in the zebrafish genome. The gene interaction network figure is from the KEGG database with modification adapted to zebrafish genes. (B) Signal intensities on the microarrays. *: q-value<0.05. **: q-value<0.01. (C) Real-time PCR results are depicted as relative abundance compared to lowest abundance sample. *: p-value<0.05. **: p-value<0.01. (D) Western blot and (E) densitometric analysis of protein samples extracted from zebrafish whole larvae shows that Socs1, Socs3a and Stat3 expression are up-regulated from 2 to 7 dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bcl2l1 WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
cish WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
jak1 WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
jak2a WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
jak2b WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
myca WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
pim1 WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
pim2 WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
ptpn6 WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
socs1a WT standard conditions Long-pec Not Detected whole organism WB
Protruding-mouth whole organism RTPCR
Protruding-mouth whole organism WB
Day 5 whole organism RTPCR
Day 5 whole organism WB
Days 7-13 whole organism WB
socs3a WT standard conditions Long-pec whole organism WB
Protruding-mouth whole organism RTPCR
Protruding-mouth whole organism WB
Day 5 whole organism RTPCR
Day 5 whole organism WB
Days 7-13 whole organism WB
spry4 WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
stat1a WT standard conditions Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
stat3 WT standard conditions Long-pec whole organism WB
Protruding-mouth whole organism WB
Day 5 whole organism WB
Days 7-13 whole organism WB
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One