ZFIN ID: ZDB-FIG-130210-8
Yin et al., 2012 - Inhibition of the pim1 oncogene results in diminished visual function. PLoS One   7(12):e52177 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Days 7-13
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

Socs3a, Socs1 and Stat3 expression in 2–7 dpf retina.

First five panels are immunohistochemical analysis showing increasing expression of Socs3a, Socs1 and Stat3 throughout the retina from 2–7 dpf. Minimal staining is observed in secondary antibody alone, pre-immune serum or morphant controls. The last three panels are in-situ hybridisations of socs3a, socs1 and stat3 genes on 2 and 7 dpf. No significant staining was observed in sense probe controls. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
socs1a WT standard conditions Long-pec lens IHC
Long-pec retinal neural layer ISH
Protruding-mouth retinal neural layer IHC
Day 5 retinal ganglion cell layer ISH
Day 5 retinal inner nuclear layer ISH
Day 5 retinal neural layer IHC
Day 5 retinal outer nuclear layer ISH
Days 7-13 retinal ganglion cell layer IHC
Days 7-13 retinal inner nuclear layer IHC
Days 7-13 retinal outer nuclear layer IHC
WT + MO3-socs1a standard conditions Long-pec Not Detected retinal neural layer IHC
socs3a WT standard conditions Long-pec eye IHC
Long-pec retinal neural layer ISH
Protruding-mouth eye IHC
Day 5 retinal ganglion cell layer ISH
Day 5 retinal inner nuclear layer ISH
Day 5 retinal neural layer IHC
Day 5 retinal outer nuclear layer ISH
Days 7-13 retinal ganglion cell layer IHC
Days 7-13 retinal inner nuclear layer IHC
Days 7-13 retinal outer nuclear layer IHC
WT + MO3-stat3 + MO5-socs3a standard conditions Long-pec Not Detected retinal neural layer IHC
stat3 WT standard conditions Long-pec eye IHC
Long-pec retinal neural layer ISH
Protruding-mouth retinal neural layer IHC
Day 5 retinal ganglion cell layer ISH
Day 5 retinal inner nuclear layer ISH
Day 5 retinal neural layer IHC
Day 5 retinal outer nuclear layer ISH
Days 7-13 retinal ganglion cell layer IHC
Days 7-13 retinal inner nuclear layer IHC
Days 7-13 retinal outer nuclear layer IHC
WT + MO3-stat3 standard conditions Long-pec Not Detected retinal neural layer IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-socs1a standard conditions Long-pec eye apoptotic, abnormal
Long-pec eye decreased size, abnormal
WT + MO3-stat3 standard conditions Long-pec eye apoptotic, abnormal
Long-pec eye decreased size, abnormal
Long-pec retinal neural layer Muller cell decreased amount, abnormal
Long-pec retinal photoreceptor layer retinal cone cell decreased amount, abnormal
WT + MO3-stat3 + MO5-socs3a standard conditions Long-pec eye apoptotic, abnormal
Long-pec eye decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One