FIGURE

Fig. 2

ID

ZDB-FIG-110224-8

Publication

Dal-Pra et al., 2011 - FoxA transcription factors are essential for the development of dorsal axial structures

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Fig. 2

Phenotype of FoxA3 morphants. (A and E) FoxA3 morphants do not present any cells expressing the hatching gland marker hgg1 at 24 hpf (E) compared to wild-type (A). (B–D, F–H). Analysis of prechordal plate (nog1 and gsc) and hatching gland (hgg1) marker expression during gastrulation in wild-type (B–D) and FoxA3 morphants (F–H). At shield stage, nog1 expression is normal in FoxA3 morphant (F). At 80% epiboly, gsc is correctly expressed in FoxA3 morphant (G), while hgg1 expression is absent (H). (I–J, M–N) foxA3 expression is increased at early (M) and late gastrula stage (N) in FoxA3 morphants compared to wild-type (I and J). (K and O) ntl is ectopically expressed in the prechordal plate of FoxA3 morphant (arrowhead, O) compared to wild-type (K) at 80% epiboly. (L and P) Injection of MO-FoxA3 prevents the formation of hatching gland in ntl^/ mutant at 30 hpf. (A, E, L, and P) Frontal views, dorsal up. (B, F, I, and M) Lateral views, animal pole up. (C, D, G, H, J, K, N, and O) Dorsal views, animal pole up.

Expression Data

Genes:	ctslb foxa3 gsc nog1 tbxta
Fish:	WT WT + MO1-foxa3 + MO2-foxa3 tbxta^{unspecified/unspecified} + MO1-foxa3 + MO2-foxa3
Knockdown Reagents:	MO1-foxa3 MO2-foxa3
Anatomical Terms:	anterior axial hypoblast axial chorda mesoderm hatching gland margin prechordal plate shield
Stage Range:	Shield to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-foxa3 + MO2-foxa3
Knockdown Reagents:	MO1-foxa3 MO2-foxa3
Observed In:	anterior axial hypoblast hatching gland notochord prechordal plate
Stage Range:	75%-epiboly to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 350(2), Dal-Pra, S., Thisse, C., and Thisse, B., FoxA transcription factors are essential for the development of dorsal axial structures, 484-495, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.