ZFIN ID: ZDB-FIG-110224-8
Dal-Pra et al., 2011 - FoxA transcription factors are essential for the development of dorsal axial structures. Developmental Biology   350(2):484-495 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 75%-epiboly to Prim-5

Fig. 2 Phenotype of FoxA3 morphants. (A and E) FoxA3 morphants do not present any cells expressing the hatching gland marker hgg1 at 24 hpf (E) compared to wild-type (A). (B–D, F–H). Analysis of prechordal plate (nog1 and gsc) and hatching gland (hgg1) marker expression during gastrulation in wild-type (B–D) and FoxA3 morphants (F–H). At shield stage, nog1 expression is normal in FoxA3 morphant (F). At 80% epiboly, gsc is correctly expressed in FoxA3 morphant (G), while hgg1 expression is absent (H). (I–J, M–N) foxA3 expression is increased at early (M) and late gastrula stage (N) in FoxA3 morphants compared to wild-type (I and J). (K and O) ntl is ectopically expressed in the prechordal plate of FoxA3 morphant (arrowhead, O) compared to wild-type (K) at 80% epiboly. (L and P) Injection of MO-FoxA3 prevents the formation of hatching gland in ntl­/­ mutant at 30 hpf. (A, E, L, and P) Frontal views, dorsal up. (B, F, I, and M) Lateral views, animal pole up. (C, D, G, H, J, K, N, and O) Dorsal views, animal pole up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ctslb WT control 75%-epiboly anterior axial hypoblast ISH
Prim-5 hatching gland ISH
WT + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly Not Detected anterior axial hypoblast ISH
Prim-5 Not Detected hatching gland ISH
tbxtaunspecified/unspecified + MO1-foxa3 + MO2-foxa3 standard conditions Prim-5 Not Detected hatching gland ISH
foxa3 WT control Shield margin ISH
Shield shield ISH
90%-epiboly prechordal plate ISH
WT + MO1-foxa3 + MO2-foxa3 standard conditions Shield margin ISH
Shield shield ISH
90%-epiboly prechordal plate ISH
gsc WT control 75%-epiboly prechordal plate ISH
WT + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly prechordal plate ISH
nog1 WT control Shield shield ISH
WT + MO1-foxa3 + MO2-foxa3 standard conditions Shield shield ISH
tbxta WT control 75%-epiboly axial chorda mesoderm ISH
75%-epiboly margin ISH
WT + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly axial chorda mesoderm ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly anterior axial hypoblast morphology, abnormal
75%-epiboly prechordal plate poorly differentiated, abnormal
Prim-5 hatching gland aplastic, abnormal
Prim-5 notochord disorganized, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 350(2), Dal-Pra, S., Thisse, C., and Thisse, B., FoxA transcription factors are essential for the development of dorsal axial structures, 484-495, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.