FIGURE

Fig. S6

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ZDB-FIG-110224-20

Publication

Dal-Pra et al., 2011 - FoxA transcription factors are essential for the development of dorsal axial structures

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Fig. S6

FoxA3 morpholinos efficiently block translation of FoxA3-GFP fusion protein. (A) Sequence of the 5′UTR and of the region coding for the Nt part of the FoxA3 protein showing the position of FoxA3 morpholinos, MO-γ1 (that covers the initiation of translation) and MO- γ3 (two nucleotides upstream of MO- γ1 in the 5′UTR part of the RNA). (B) Injection of 50 pg of RNA coding for a FoxA3-GFP fusion protein at the 4 cell stage results in embryo displaying a strong fluorescence in the nucleus of one quarter of the cells. (C) Injection of 8 ng of both MO- γ1 and MO- γ3 as well as (D) 8 ng of MO- γ1 or (E) 8 ng of MO- γ3 abolish the translation of FoxA3-GFP fusion protein (central panels in C–E compared to right panel in A). Morpholinos have been injected with a RNA coding for a RFP used as control to show that all embryos observed are morphants (right panels in C–E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 350(2), Dal-Pra, S., Thisse, C., and Thisse, B., FoxA transcription factors are essential for the development of dorsal axial structures, 484-495, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.