PUBLICATION

FoxA transcription factors are essential for the development of dorsal axial structures

Authors
Dal-Pra, S., Thisse, C., and Thisse, B.
ID
ZDB-PUB-101222-49
Date
2011
Source
Developmental Biology   350(2): 484-495 (Journal)
Registered Authors
Dal-Pra, Sophie, Thisse, Bernard, Thisse, Christine
Keywords
FoxA, gastrula, notochord, floor plate, hypochord
MeSH Terms
  • Animals
  • Forkhead Transcription Factors/physiology*
  • Gastrulation
  • Hepatocyte Nuclear Factor 3-gamma/physiology*
  • Mesoderm/physiology
  • Zebrafish/embryology*
  • Zebrafish Proteins/physiology*
PubMed
21172337 Full text @ Dev. Biol.
Abstract
In vertebrates, embryonic structures present at the dorsal midline, prechordal plate, notochord, hypochord and floor plate, share a common embryonic origin. In zebrafish, they derive from a pool of progenitors located within the embryonic shield at the onset of gastrulation. The molecular mechanisms responsible for the common development of these structures remain unknown. Based on their spatial and temporal expression, transcription factors of the Forkhead box A (FoxA) family appeared to be good candidates to play such a role. In agreement with this hypothesis, we found that simultaneous knockdown of FoxA2 and FoxA3 abolish the formation of all axial derivatives while over-expression of these transcription factors strongly enlarges dorsal mesodermal territories. We establish that, in FoxA2-FoxA3 double morphants, precursors of axial tissues are correctly induced at early gastrula stage, but their dorsal midline identity is not maintained during development and we found that progenitors of these tissues are cell-autonomously re-specified to form muscle fibers as well as cells of the ventral neural tube. Our study provide the first example of a specific loss of all dorsal midline tissues and demonstrates that members of the FoxA family have redundant functions essential to maintain the axial identity of prechordal plate, notochord, floor plate and hypochord progenitors during gastrulation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes