ZFIN ID: ZDB-FIG-101101-16
Kwon et al., 2009 - Mesendodermal signals required for otic induction: Bmp-antagonists cooperate with Fgf and can facilitate formation of ectopic otic tissue. Developmental dynamics : an official publication of the American Association of Anatomists   238(6):1582-1594 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 3 Formation of preplacodal ectoderm and various cranial placodes in chd/fsta/cv2 triple-morphants. A, B:six4.1 expression at 10.5 hpf in a wild-type control (A) and chd/fsta/cv2 triple-morphant (B). C, D:eya1 expression at 10.5 hpf in a wild-type control (C) and chd/fsta/cv2 triple-morphant (D). E, F:dlx3b expression at 10.5 hpf in a wild-type control (E) and chd/fsta/cv2 triple-morphant (F). G, H:pitx3 expression at 19 hpf in a wild-type control (G) and chd/fsta/cv2 triple-morphant (H). The anterior pituitary (ap), lens placode (lp), and trigeminal placode (tp) are indicated. I, J:cxcr4b expression at 19 hpf in a wild-type control (I) and chd/fsta/cv2 triple-morphant (J). The nasal placode (np) and trigeminal placode (tp) are indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cxcr4b WT standard conditions 20-25 somites olfactory placode ISH
20-25 somites trigeminal ganglion ISH
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions 20-25 somites olfactory placode ISH
20-25 somites trigeminal ganglion ISH
dlx3b WT standard conditions Bud preplacodal ectoderm ISH
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions Bud preplacodal ectoderm ISH
eya1 WT standard conditions Bud preplacodal ectoderm ISH
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions Bud preplacodal ectoderm ISH
pitx3 WT standard conditions 20-25 somites adenohypophyseal placode ISH
20-25 somites solid lens vesicle ISH
20-25 somites trigeminal ganglion ISH
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions 20-25 somites adenohypophyseal placode ISH
20-25 somites solid lens vesicle ISH
20-25 somites Not Detected trigeminal ganglion ISH
six4b WT standard conditions Bud preplacodal ectoderm ISH
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions Bud preplacodal ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions 20-25 somites trigeminal placode decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.