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ZFIN ID: ZDB-FIG-101101-17
Kwon et al., 2009 - Mesendodermal signals required for otic induction: Bmp-antagonists cooperate with Fgf and can facilitate formation of ectopic otic tissue. Developmental dynamics : an official publication of the American Association of Anatomists   238(6):1582-1594 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 1-4 somites to 5-9 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 1-4 somites to Prim-5

Fig. 4 Partial loss of Nodal and Fgf signaling results in medial expansion of otic tissue. In the indicated columns, embryos were treated with 70 μM SB431542 beginning at 0.5 hpf (nodal ↓). A–C: Otic vesicles at 28 hpf in wild-type embryos (A, C), and an fgf8+/- heterozygote (B). D, E: Expression of ear markers dlx3b and pax5 (blue) and the hindbrain marker krox20 (red) at 28 hpf in a wild-type control (D) and a wild-type embryo treated with 1 μM SU5402 beginning at 5.5 hpf (E). F–H: Expression of foxi1 at 11 hpf in wild-type embryos (F, H) and an fgf8+/- heterozygote (G, 2/42 affected). I–K: Expression of dlx3b at 11.5 hpf in wild-type embryos (I, K) and an fgf8+/- heterozygote (J, 4/56 affected). L–N: Expression of pax2a at 12 hpf in wild-type embryos (L, N) and an fgf3+/- heterozygote (M, 7/87 affected). O–Q: Expression of pax8 and shh in wild-type embryos (O, Q) and an fgf8+/- heterozygote (P, 4/51 affected). R–T: Expression of fsta (blue) and krox20 (red) at 11 hpf in wild-type embryos (R, T) and an fgf8+/- heterozygote (S, 40/52 affected). U–W: Expression of islet1 in the trigeminal placode at 11 hpf in wild-type embryos (U, W) and an fgf8+/- heterozygote (V, 3/46 affected). All images show dorsal views (anterior to top). Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT standard conditions 1-4 somites otic placode ISH
WT chemical treatment: SB 431542 1-4 somites otic placode ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites otic placode ISH
egr2b WT standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
WT chemical treatment: SB 431542 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
foxi1 WT standard conditions 1-4 somites otic placode ISH
WT chemical treatment: SB 431542 1-4 somites otic placode ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites otic placode ISH
fsta WT standard conditions 1-4 somites paraxial mesoderm ISH
WT chemical treatment: SB 431542 1-4 somites paraxial mesoderm ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites paraxial mesoderm ISH
isl1a WT standard conditions 1-4 somites trigeminal placode ISH
WT chemical treatment: SB 431542 1-4 somites trigeminal placode ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites trigeminal placode ISH
pax2a WT standard conditions 5-9 somites otic placode ISH
WT chemical treatment: SB 431542 5-9 somites otic placode ISH
fgf8ati282a/+ chemical treatment: SB 431542 5-9 somites otic placode ISH
pax8 WT standard conditions 1-4 somites otic placode ISH
WT chemical treatment: SB 431542 1-4 somites otic placode ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites otic placode ISH
shha WT standard conditions 1-4 somites axial mesoderm ISH
WT chemical treatment: SB 431542 1-4 somites Not Detected axial mesoderm ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites Not Detected axial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: SB 431542 1-4 somites paraxial mesoderm mislocalised medially, abnormal
Prim-5 otic vesicle decreased size, abnormal
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites otic placode decreased amount, abnormal
1-4 somites otic placode formation process quality, abnormal
1-4 somites otic placode malformed, abnormal
1-4 somites otic placode mislocalised medially, abnormal
1-4 somites paraxial mesoderm mislocalised medially, abnormal
Prim-5 otic vesicle decreased amount, abnormal
Prim-5 otic vesicle increased size, abnormal
Prim-5 otic vesicle malformed, abnormal
Prim-5 otic vesicle mislocalised medially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.