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ZFIN ID: ZDB-FIG-101101-14
Kwon et al., 2009 - Mesendodermal signals required for otic induction: Bmp-antagonists cooperate with Fgf and can facilitate formation of ectopic otic tissue. Developmental dynamics : an official publication of the American Association of Anatomists   238(6):1582-1594 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 1 Loss of mesendoderm and fgf8 or fgf3 disrupts otic induction. Lateral views (anterior to left) of otic vesicles in live embryos at 35 hpf. A: Wild-type control. B: Wild-type embryo treated with 100 μM SB431542. C:fgf8-/- mutant. D,E:fgf8-/- mutants treated with 100 μM SB431542 showing either a micro-vesicle (D, arrow) or no morphologically detectable vesicle (E, asterisk). F:fgf3-morphant embryo treated with 100 μM SB431542 showing no morphologically detectable otic vesicle (asterisk). Scale bar = 25 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: SB 431542 Prim-15 otic vesicle decreased size, abnormal
Prim-15 otic vesicle malformed, abnormal
WT + MO8-fgf3 chemical treatment: SB 431542 Prim-15 otic vesicle agenesis, abnormal
Prim-15 otic vesicle formation process quality, abnormal
fgf8ati282a/ti282a standard conditions Prim-15 otic vesicle decreased size, abnormal
Prim-15 otic vesicle malformed, abnormal
fgf8ati282a/ti282a chemical treatment: SB 431542 Prim-15 otic vesicle agenesis, abnormal
Prim-15 otic vesicle formation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.